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Efficient preparation method of low-value fish immunomdodulating peptides

A technology of immune regulation and low-value fish, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc., to achieve the effect of improving yield and activity

Active Publication Date: 2019-08-02
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This technology allows for efficient production of antimicrobial agents that have improved effectiveness over existing methods such as chemical or physical techniques. It involves treatings fish muscle proteins before they enter into humans through injection. By doing this treatment, these molecules become more effective at killing germs on their own without harmful side reactions from other substances used during chemotherapy. These processes involve extracting certain types of polypeptidic compounds called chitinosine (C14H22N4) therefrom and then subject them to various forms of electrophoresis. Afterwards, it'll separate different types of C-14 hydrolyzed chitosan gum protein fragments containing amino acids like lysines and histamine onto another gel filtrate afterward.

Problems solved by technology

This patents describes how it takes advantage over existing technologies like chemical synthesis techniques when developing new compounds called metabolites due to their unique properties. These novel processes involve combining ingredients together without causing them apart too much, allowing for effective extraction of these substances. Additionally, previous researchers found that adding ionizable lipophilics could enhance absorption of neurotransmitters within brain regions involved in nerve function. By doing this, we may develop innovatives against current drug therapies based upon chemotherapy alone.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0024] Mix and homogenize golden thread fish myosin and 0.3Mmol NaCl (pH=6.8) at a concentration ratio of 1:10, put the homogenized myosin solution into a high-pressure micro-jet nano-homogenizer, and put the high-pressure nozzle The pore diameter was set to 5mm, the control treatment temperature was 35°C, the pressure of high-pressure homogenization treatment was 100MPa, the treatment time was 10s, and the number of cycles was 5 times. The mass ratio of protease to protein homogenate is 1:20, add trypsin (Wuxi Genencor Bioengineering Co., Ltd., 250u / mg), enzymolysis pH=7.5, enzymolysis temperature 40°C, stop enzymolysis after 60min, enzymolysis solution 90 ℃ water bath for 15 minutes to kill the enzyme; the enzymatic solution was centrifuged to remove the precipitate and then placed in the pulse electric field processor, connected to the ultrafiltration membrane, set the electromagnetic wave wavelength to 0.5cm, the electric field frequency to 4000MHz, and the radiation power ...

Embodiment 2

[0026] Mix and homogenize the myosin from golden thread fish with 0.3Mmol NaCl (pH=6.8) at a concentration ratio of 1:10, put the homogenized myosin solution into a high-pressure micro-jet nano-homogenizer, and put the high-pressure The diameter of the nozzle was set to 5mm, the control treatment temperature was 35°C, the pressure of high-pressure homogenization treatment was 150MPa, the treatment time was 20s, and the number of cycles was 5 times. Under these conditions, the high-pressure homogenization treatment was carried out on the myosin solution of golden thread fish. Then add trypsin (Wuxi Genencor Bioengineering Co., Ltd., 250u / mg) at a mass ratio of trypsin to protein homogenate of 1:30, enzymatic hydrolysis pH=7.5, enzymatic hydrolysis temperature 40°C, stop enzymatic hydrolysis after 90min, enzymatic Inactivate the enzyme in a water bath at 90°C for 15 minutes. The enzymolysis solution was centrifuged to remove the precipitate and then placed in the pulse electric ...

Embodiment 3

[0028]Mix and homogenize the myosin from golden thread fish with 0.3Mmol NaCl (pH=6.8) at a concentration ratio of 1:10, put the homogenized myosin solution into a high-pressure micro-jet nano-homogenizer, and put the high-pressure The aperture of the nozzle was set at 5mm, the treatment temperature was controlled at 35°C, the pressure of high-pressure homogenization treatment was 200MPa, the treatment time was 30s, and the number of cycles was 5 times. Under these conditions, the high-pressure homogenization treatment was performed on the myosin solution of golden thread fish. Then add trypsin (Wuxi Genencor Bioengineering Co., Ltd., 250u / mg) at a mass ratio of trypsin to protein homogenate of 1:40, enzymatic hydrolysis pH=7.5, enzymatic hydrolysis temperature 40°C, stop enzymatic hydrolysis after 1200min, enzymatic Inactivate the enzyme in a water bath at 90°C for 15 minutes. The enzymolyzed solution was centrifuged to remove the precipitate and then placed in the pulse elec...

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Abstract

The invention discloses an efficient preparation method of low-value fish immunomdodulating peptides, relates to the development and utilization of low-value fish bioactive peptides and related anti-inflammatory drug products, and belongs to the technical field of low-value fish processing; the preparation method comprises the steps of preparing nemipterus virgatus myosin by a salt extraction method, fully expanding a high-spiral structural domain of myofibrillar protein by using a high-pressure homogenization method; fine-tuning a protein solution to pH 7.5, adding trypsin, performing enzymatic hydrolysis for 90 minutes, promoting an ultrafiltration membrane to separate a nemipterus virgatus protein enzymatic hydrolysate by using impressed current, and obtain a peptide fragment of 500 to3000 components; finally obtaining a powdery product by a freeze-drying method; the method can improve the degree of hydrolysis of the nemipterus virgatus myosin peptide, and the biological propertiesand yield of the immunomodulating peptides; the anti-inflammatory activity of the immunomodulatory peptides prepared by the method is better; the invention realizes the deep processing and comprehensive utilization of the low-value fish, and has important meaning for improving the added value of the low-value fish.

Description

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Claims

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Application Information

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Owner FUJIAN AGRI & FORESTRY UNIV
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