Method for repairing oil-contaminated soil through reinforcement of biochar-base complex microbial inoculant

A compound bacterial agent, oil pollution technology, applied in the restoration of polluted soil and other directions, can solve the problems of slow growth of oil-degrading microorganisms, reduced survival rate, resource consumption, etc., and achieves easy control of process parameters, guaranteed degradation efficiency, and degradation quality. Guaranteed effect

Inactive Publication Date: 2019-08-13
YONKER ENVIRONMENTAL PROTECTION
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of degrading petroleum-contaminated soil or oil sludge in this method, free bacterial liquid is used as bacterial agent. Since free bacterial agent is easily affected by the environment in the polluted medium, the growth rate is slow, the survival rate is reduced, and the oil degradation performance is also eas

Method used

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  • Method for repairing oil-contaminated soil through reinforcement of biochar-base complex microbial inoculant
  • Method for repairing oil-contaminated soil through reinforcement of biochar-base complex microbial inoculant
  • Method for repairing oil-contaminated soil through reinforcement of biochar-base complex microbial inoculant

Examples

Experimental program
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Effect test

Example Embodiment

[0033] (1) Preparation of biochar-based composite bacterial agent: Biochar is used as a carrier to prepare Mycobacterium CSC-6 bacterial liquid by adsorption method; the preservation number of Mycobacterium CSC-6 is CCTCC No: M 2017726, which is a typical culture in China Preserved by the collection center, the preservation date is November 27, 2017; the biochar can be a mixture of one or more of rice husks, cotton stalks, japonica rice or rattan.

[0034] (2) preparation of biosurfactant: taking mannitol as carbon source, fermenting and culturing Pseudomonas aeruginosa W-3 to obtain a fermentation broth containing biosurfactant, and this biosurfactant is rhamnolipid ; The preservation number of Pseudomonas aeruginosa W-3 is CCTCC NO: M2010048, which is preserved in the China Center for Type Culture Collection, and the preservation date is March 5, 2010.

[0035] (3) Conservation of polluted soil: crush the polluted soil, sieve the polluted soil with a sample sieve with a fine...

Example Embodiment

[0040] Example 1

[0041] The present embodiment adopts the method of strengthening the restoration of oil-contaminated soil by using the biochar-based composite inoculant to treat the fresh oil-contaminated soil, and the test method is as follows:

[0042] (1) Collect uncontaminated fresh soil, crush it and pass through a 10-mesh sample sieve, add oil to make the oil content 30,000 mg / kg, and obtain fresh oil-contaminated soil with an oil content of 30,000 mg / kg;

[0043] (2) Using biochar as a carrier to prepare Mycobacterium CSC-6 bacterial liquid by adsorption method, wherein the mass fraction of Mycobacterium CSC-6 is 1%, and the biochar is selected from rice husk charcoal; using mannitol as a carbon source, the Pseudomonas aeruginosa W-3 is fermented and cultured to obtain a fermentation broth containing a biosurfactant, and diluted with water to obtain a dilution of the fermentation broth containing a biosurfactant, wherein the biosurfactant is rhamnolipid, and the bios...

Example Embodiment

[0047] Example 2

[0048] The present embodiment adopts the method of strengthening the restoration of oil-contaminated soil by using the biochar-based composite inoculant to treat the fresh oil-contaminated soil, and the test method is as follows:

[0049] (1) Collect uncontaminated fresh soil, crush it and pass through a 10-mesh sample sieve, add oil to make the oil content 50,000 mg / kg, and obtain fresh oil-contaminated soil with an oil content of 50,000 mg / kg;

[0050] (2) Using biochar as a carrier to prepare Mycobacterium CSC-6 bacterial liquid by adsorption method, wherein the mass fraction of Mycobacterium CSC-6 is 1%, and the biochar is selected from rice husk charcoal; using mannitol as a carbon source, the Pseudomonas aeruginosa W-3 is fermented and cultured to obtain a fermentation broth containing a biosurfactant, and diluted with water to obtain a dilution of the fermentation broth containing a biosurfactant, wherein the biosurfactant is rhamnolipid, and the bios...

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Abstract

The invention relates to a method for repairing oil-contaminated soil through reinforcement of a biochar-base complex microbial inoculant. The method includes the following steps of preparing a mycobacterium CSC-6 bacteria solution through an adsorption method with biochar as the carrier; conducting fermentation culture on pseudomonas aeruginosa W-3 with mannitol as the carbon source to obtain a fermentation liquid with a biosurfactant; curing contaminated soil, wherein after the contaminated soil is broken and sieved, a compound fertilizer is added, a diluent containing the fermentation liquid with the biosurfactant is added, the water content of the soil is adjusted to be 20-50%, a porous membrane is applied, and curing is conducted for 1-2 weeks at 20-30 DEG C; degrading the contaminated soil, wherein the biochar-base complex microbial inoculant is added to the cured contaminated soil, plowing is conducted once every 1-2 weeks, and the water content of the soil is adjusted to be 20-50%. Compared with the prior art, the method has the advantages of being high in petroleum degradation efficiency, high in degradation speed, simple in process, low in cost and the like.

Description

technical field [0001] The invention relates to a treatment method for oil sludge and oil-polluted soil, in particular to a method for strengthening and repairing oil-polluted soil with a biochar-based composite bacterial agent. Background technique [0002] Oil exploration and refining industries produce a large amount of oily sludge. Due to historical reasons, there is a lack of a sound regulatory system and treatment technology. Oil sludge is usually disposed of in landfills and piles without treatment, leaving serious sludge pollution problem. If oily sludge is not disposed of in a timely and appropriate manner, it will have varying degrees of impact on the surrounding environment. For example, the volatilization of oil and gas in oily sludge will pollute the air and cause great harm to the health of surrounding residents; the piled oily sludge will pollute surface water and even groundwater, which has potential health risks to humans; and a large amount of oily sludge ...

Claims

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Application Information

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IPC IPC(8): B09C1/10B09C1/00
CPCB09C1/00B09C1/10
Inventor 闫淑兰罗启仕崔长征任静赵秀红李梓
Owner YONKER ENVIRONMENTAL PROTECTION
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