A kind of supramolecular polypeptide with self-assembled aggregate structure and preparation method thereof

A self-assembly and aggregation technology, applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve the problems of single function, low effective utilization rate, and safety to be studied, and achieves mild process conditions and realizes Industrial production, excellent slow release effect

Active Publication Date: 2022-07-15
上海璞萃生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many kinds of peptide products on the market, but their functions are relatively single. Generally, the peptide solution is directly added to the formula, and its effective utilization rate is generally low, and safety issues still need to be studied

Method used

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  • A kind of supramolecular polypeptide with self-assembled aggregate structure and preparation method thereof
  • A kind of supramolecular polypeptide with self-assembled aggregate structure and preparation method thereof
  • A kind of supramolecular polypeptide with self-assembled aggregate structure and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A supramolecular polypeptide with a self-assembled aggregate structure is formed by the self-assembly of an organic carboxylate-polypeptide two-component complex. Micron-scale self-assembly.

[0026] Further, the polypeptide is palmitoyl tetrapeptide-7, acetyl hexapeptide-8, palmitoyl tripeptide-1, acetyl tetrapeptide-5, palmitoyl pentapeptide-4, snake venom-like peptide, nonapeptide-1 , at least one of carnosine.

[0027] Further, the organic carboxylate is at least one of sodium laurate, sodium palmitate, sodium stearate, sodium oleate, sodium arachidonic acid, sodium behenate, sodium hyaluronate, and sodium alginate.

[0028] A kind of preparation method of the supramolecular polypeptide with self-assembled aggregate structure, its technology comprises the steps:

[0029] Step 1. Dissolve the polypeptide in a solvent with a pH value of 5-8, and the concentration of the polypeptide is 0.01-100 mg / mL;

[0030] Step 2, adding organic carboxylate to the solution forme...

Embodiment 2

[0034] A kind of preparation method of the supramolecular polypeptide with self-assembled aggregate structure, its technology comprises the steps:

[0035] Step 1. Add palmitoyl tetrapeptide-7 into a 100 mL round-bottomed flask equipped with a magnetic stirring bar, and then add sodium acetate buffer with a pH value of 5.0 to it to form a mixed solution. The palmitoyl tetrapeptide-7 is in The concentration in the mixture is 10 mg / mL;

[0036] Step 2. Put the round-bottomed flask with the mixed solution on a constant temperature water bath magnetic stirrer, the temperature of the water bath is 70°C, then add sodium laurate to it, stir at a rotational speed of 300r / min for 4h, and cool to 10°C Then, supramolecular polypeptides are obtained. The mixed solution after the reaction was centrifuged at 3000 r / min for 5 min, the supernatant was discarded, the obtained precipitate was washed three times with distilled water, and then freeze-dried into powder, and the obtained powder wa...

Embodiment 3

[0038] A kind of preparation method of the supramolecular polypeptide with self-assembled aggregate structure, its technology comprises the steps:

[0039] Step 1. Add acetyl hexapeptide-8 into a 100 mL round-bottomed flask equipped with a magnetic stirrer, and then add sodium acetate buffer with a pH value of 5.0 to it to form a mixed solution. The acetyl hexapeptide-8 is in The concentration in the mixture is 30mg / mL;

[0040] Step 2. Put the round-bottomed flask containing the mixed solution on a constant temperature water bath magnetic stirrer, the temperature of the water bath is 70°C, then add sodium palmitate to it, stir at a rotational speed of 500r / min for 6h, and cool to 10°C Then, supramolecular polypeptides are obtained. The reaction mixture was centrifuged at 5000 r / min for 10 min, the supernatant was discarded, the obtained precipitate was washed three times with distilled water, and then freeze-dried into powder, and the obtained powder was sealed and stored at...

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Abstract

The invention provides a supramolecular polypeptide with a self-assembled aggregate structure, which is formed by self-assembly of an organic carboxylate-polypeptide two-component complex, and the organic carboxylate and the polypeptide are aggregated through hydrophobic interaction and electrostatic attraction. into nano- and micro-scale self-assembly. The present invention provides a supramolecular polypeptide with a self-assembled aggregate structure. The intermolecular force in the supramolecular polypeptide is a non-covalent bond force, which can slowly release the polypeptide. After using the supramolecular polypeptide of the present invention, The polypeptide concentration on the skin surface can be maintained at a high level for at least 12 hours, and the sustained release effect is excellent; the process conditions of the method are very mild, and the structure of the polypeptide itself will not be damaged, so the biological activity of the polypeptide can be guaranteed; The process is simple, and it is easy to realize industrial production.

Description

technical field [0001] The invention relates to the field of cosmetics, in particular to a supramolecular polypeptide with a self-assembled aggregate structure and a preparation method thereof. Background technique [0002] The skin is the protector of the internal organs and tissues of the human body, and is also the effector of the internal organs, the spirit and the surrounding environment. It plays a particularly important role in the health and beauty of the human body. Active biological peptides play an important physiological role in the natural aging defense and nursing process of skin tissue cells such as proliferation, cell chemotaxis and migration, repair and regeneration, angiogenesis and reconstruction, pigment formation and clearance, protein synthesis and secretion, etc. . [0003] Polypeptide is a natural active substance formed by covalently linking two or more amino acids through peptide bonds. It is homologous to body substances, has a small molecular wei...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/64A61K8/36A61K8/73A61Q19/00
CPCA61K8/64A61K8/361A61K8/733A61K8/735A61Q19/00
Inventor 王璞
Owner 上海璞萃生物科技有限公司
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