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Bacteria inhibitor and preparation method thereof

A bacteriostatic agent and seed technology, which is applied in the field of fermentation technology and microorganisms, can solve problems such as easy to cause human body, weak antibacterial effect, allergic reaction, etc., achieve strong antibacterial activity, small diarrhea effect, and relieve diarrhea effect Effect

Inactive Publication Date: 2019-08-16
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, commonly used bacteriostatic agents include Cathone, paraben esters, benzoic acid, sodium benzoate, sorbic acid and salicylic acid, etc., wherein, Cathone and paraben esters have stronger antibacterial effects, but, Cathone and paraben esters are more irritating to the human body and can easily cause allergic reactions in the human body; benzoic acid, sodium benzoate, sorbic acid and salicylic acid are less harmful to the human body, but benzoic acid, sodium benzoate, sorbic acid And the antibacterial effect of salicylic acid is weak, they all have obvious defects in practical application
[0004] Some studies have also found some new antibacterial agents with strong antibacterial effect and less harm to the human body, such as resveratrol. However, resveratrol needs to be extracted from nature, which has the disadvantages of difficult extraction and small yield. , there are also some difficulties in practical application
[0005] Therefore, there is an urgent need to find a bacteriostatic agent with strong antibacterial effect, less harm to the human body, easy production and large output to solve the problems existing in the existing bacteriostatic agents.

Method used

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  • Bacteria inhibitor and preparation method thereof
  • Bacteria inhibitor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Specific steps are as follows:

[0038] Apply Monascus purpureus spores on the PDA slant medium, activate at 30°C for 7 days to obtain activated spores; scrape off the activated spores on the PDA slant medium, and resuspend to the spore concentration with distilled water 1×10 7 individual / mL to obtain the spore liquid; the spore liquid was inoculated into the seed medium according to the inoculum amount of 5% (v / v), and cultivated for 2 days at a temperature of 30° C. to obtain the seed liquid; the seed liquid was inoculated according to 10% ( The inoculation amount of v / v) was inoculated into rhubarb medium containing 40g rhubarb powder (30 mesh particle size), 15g glucose, 5g yeast powder, 10mL soybean hydrolyzate and 30mL water, and fermented for 20d under the condition of temperature 30°C, The fermented product is obtained; the fermented product is vacuum freeze-dried and crushed to obtain the antibacterial agent 1.

Embodiment 2

[0040] Specific steps are as follows:

[0041] Apply Monascus purpureus spores on the PDA slant medium, activate at 30°C for 7 days to obtain activated spores; scrape off the activated spores on the PDA slant medium, and resuspend to the spore concentration with distilled water 1×10 7 individual / mL to obtain the spore liquid; the spore liquid was inoculated into the seed medium according to the inoculation amount of 5% (v / v), and cultivated for 2 days under the condition of a temperature of 30° C. to obtain the seed liquid; the seed liquid was inoculated according to 5% ( The inoculation amount of v / v) was inoculated into the rhubarb medium containing 40g rhubarb powder (particle size 40 mesh), 5g glucose, 15g maltose, 10g yeast powder and 30mL water, and fermented at a temperature of 30°C for 15 days to obtain a fermented The fermented product was vacuum freeze-dried and crushed to obtain the antibacterial agent 2.

Embodiment 3

[0043] Specific steps are as follows:

[0044] Apply Monascus purpureus spores on the PDA slant medium, activate at 30°C for 7 days to obtain activated spores; scrape off the activated spores on the PDA slant medium, and resuspend to the spore concentration with distilled water 1×10 7individual / mL to obtain the spore liquid; the spore liquid was inoculated into the seed medium according to the inoculation amount of 5% (v / v), and cultivated for 2 days under the condition of a temperature of 30° C. to obtain the seed liquid; the seed liquid was inoculated according to 7% ( The inoculation amount of v / v) was inoculated into the rhubarb medium containing 40g rhubarb powder (particle size 40 mesh), 5g glucose, 15g maltose, 10g yeast powder and 60mL water, and fermented for 35d under the condition of temperature 25°C to obtain the fermented The fermented product was vacuum freeze-dried and pulverized to obtain the antibacterial agent 3.

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Abstract

The invention discloses a bacteria inhibitor and a preparation method thereof, and belongs to the technical field of microorganisms and fermentation. Rheum officinale is rich in anthraquinone compounds such as rhein, emodin, physcion, aloe-emodin and the like, and the compounds can hinder the synthesis of nucleic acid and protein of bacterial cells, therefore, the rheum officinale has an antibacterial effect, broad antibacterial spectrum and a strong effect. However, the anthraquinone compounds in the rheum officinale are mostly in a combined form, and the combined anthraquinone can cause therheum officinale to have a strong purgative effect and is more harmful to human bodies, therefore, the rheum officinale is not suitable to be used as an antibacterial agent in products in contact withhuman bodies such as foods, medicines, cosmetics and the like. The rheum officinale is fermented by monascus, so that the content of combined anthraquinone in the rheum officinale is greatly reduced,the purgative effect is relieved, and meanwhile, the antibacterial activity is enhanced.

Description

technical field [0001] The invention relates to a bacteriostatic agent and a preparation method thereof, belonging to the technical field of microbes and fermentation technology. Background technique [0002] Antibacterial agent is a kind of substance that can inhibit the growth of bacteria, which can delay the deterioration and spoilage of food, medicine, cosmetics and other products, and can also be used to treat some diseases caused by bacteria. have important applications. [0003] At present, commonly used bacteriostatic agents include Cathone, paraben esters, benzoic acid, sodium benzoate, sorbic acid and salicylic acid, etc., wherein, Cathone and paraben esters have stronger antibacterial effects, but, Cathone and paraben esters are more irritating to the human body and can easily cause allergic reactions in the human body; benzoic acid, sodium benzoate, sorbic acid and salicylic acid are less harmful to the human body, but benzoic acid, sodium benzoate, sorbic acid ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/708A61K36/899A61P31/04A61P35/00A61P39/02A61P3/06A61P39/06A61P9/12A61P3/10
CPCA61K36/062A61K36/708A61K36/899A61K2236/19A61P3/06A61P3/10A61P9/12A61P31/04A61P35/00A61P39/02A61P39/06A61K2300/00
Inventor 夏小乐段亮杰许睿显李冉
Owner JIANGNAN UNIV
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