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Non-coding RNA-LOC105376991 for diagnosing male osteoporosis

An osteoporosis, long-chain non-coding technology, applied in the field of biomedicine, can solve the problems of little help in early diagnosis of osteoporosis, low sensitivity and accuracy of osteoporosis, etc.

Inactive Publication Date: 2019-08-20
GUAN BOJIAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, plain X-ray films have low sensitivity and accuracy in diagnosing osteoporosis, and are of little help in the early diagnosis of osteoporosis.

Method used

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  • Non-coding RNA-LOC105376991 for diagnosing male osteoporosis
  • Non-coding RNA-LOC105376991 for diagnosing male osteoporosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Screening of differentially expressed molecules

[0053] 1. Case collection

[0054] Five elderly male patients with osteoporosis, aged 62-79 years old, were selected; the diagnosis of osteoporosis was based on the standard set by the Osteoporosis Committee of the Chinese Geriatrics Society, that is, the BMD value at the same location was lower than the peak value in the region by two standard deviations Defined as osteoporosis. During the same period, 6 healthy elderly men (healthy control group), aged 61-78 years, were selected. Eligible subjects excluded diabetes, primary hyperparathyroidism and hyperthyroidism, hypercortisolism, liver and kidney dysfunction, alcoholism, malnutrition, rheumatoid arthritis and other diseases affecting bone metabolism; no application within six months Androgens, calcitonin, large amounts of calcium, bisphosphonates, and other drugs that affect bone metabolism. The two groups were comparable in terms of age, height, body ma...

Embodiment 2

[0095] Example 2 The differentially expressed LncRNA screened out by large sample verification

[0096] 1. Sample collection

[0097] According to the method of Example 1, 32 osteoporosis patients and 30 healthy male controls were collected.

[0098] 2. Extraction of total RNA from blood

[0099]The steps are the same as in Example 1.

[0100] 3. Validation at the RNA level

[0101] (1) Reverse transcription: According to the measured RNA concentration, 1000 ng of RNA was quantified for each sample for reverse transcription. Use Dalian Biobio's DDR037A reverse transcription kit, 20 μl of the system (see Table 1), add it to a 200 μl de-enzyme EP tube, centrifuge and place it in a PCR instrument, the temperature conditions are set as: 37 ° C for 15 min, 85 ° C for 5 sec . After the end, the cDNA was diluted 10 times and stored at -20°C for future use.

[0102] Table 1 Reverse transcription system

[0103]

[0104] (2) Real-time quantitative PCR

[0105] Shanghai Sango...

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Abstract

The invention discloses a diagnosing product for male osteoporosis. The diagnosing product achieves the diagnosing aim by detecting LOC105376991 in blood. Researches prove that the level of the LOC105376991 in the blood of a male osteoporosis patient is remarkably reduced in comparison with that of a normal male. According to the correlation existing between the LOC105376991 and the male osteoporosis, a kit for diagnosing male osteoporosis can be prepared and widely applied clinically.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a non-coding RNA for diagnosing male osteoporosis, the non-coding RNA is LOC105376991. Background technique [0002] The incidence of osteoporosis in men is much lower than that in postmenopausal women, so in the past, the importance of osteoporosis in men was far less than that of women. Research results in recent years have shown that although young men can achieve higher peak bone mass than women, the onset of bone mass loss is also significantly later than that of women, but both men and women have a bone loss phase with age. process, namely senile osteoporosis (type II). There are many similarities between male osteoporosis and female osteoporosis, but there are still significant gender differences in etiology, pathology, and etiology. Osteoporosis of varying degrees is common in men over the age of 65, and its serious complication is fractures. [0003] In the United States, the...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6837C12N15/113C40B40/06
CPCC12Q1/6837C12Q1/6883C12Q2600/178C40B40/06
Inventor 杨承刚向常娟
Owner GUAN BOJIAN BIOTECH CO LTD