Method for determining folic acids in blood spots through high performance liquid chromatography-tandem mass spectrometry

A technology of high performance liquid chromatography combined with mass spectrometry, applied in the field of high performance liquid chromatography-tandem mass spectrometry for the determination of folic acid in blood spots, can solve the problems of complex matrix of blood spot samples, achieve simple pretreatment methods, less blood collection Significant economic benefits

Inactive Publication Date: 2019-08-20
上海可力梅塔生物医药科技有限公司 +3
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the complex matrix of blood spot samples, it needs to be extracted for detection

Method used

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  • Method for determining folic acids in blood spots through high performance liquid chromatography-tandem mass spectrometry
  • Method for determining folic acids in blood spots through high performance liquid chromatography-tandem mass spectrometry
  • Method for determining folic acids in blood spots through high performance liquid chromatography-tandem mass spectrometry

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Experimental program
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Effect test

Embodiment 1

[0045] 1. Sample collection

[0046] (1) Because food affects the measurement results, the subjects need to take samples on an empty stomach, and the blood spot collectors wash their hands and wear sterile gloves;

[0047] (2) The blood was collected and dropped on the filter paper made of folic acid, so that the blood naturally penetrated to the back of the filter paper, avoiding repeated bleeding, and at least 3 blood spots were collected;

[0048] (3) Place the blood spots in the air and let them dry naturally until dark brown, avoiding sunlight and ultraviolet radiation, baking, volatile chemical substances and other pollution;

[0049] (4) All blood spots should be treated as specimens of blood-borne infectious diseases, and specimens of special infectious diseases, such as AIDS, should be indicated and packaged separately;

[0050] (5) Qualified dried blood spots should be: at least 3 blood spots, and the diameter of each blood spot is greater than 8 mm; the blood dropl...

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Abstract

The invention provides a method for determining folic acids in blood spots through high performance liquid chromatography-tandem mass spectrometry. The folic acid and 5-methyltetrahydrofolate are separated by high performance liquid chromatography, and then mass spectrometry isotope internal standard quantitation is used. The concentration of the standard is taken as the X-axis, and the peak arearatio of the standard to the internal standard is the Y-axis to establish a calibration curve. The content of the folic acid and 5-methyltetrahydrofolate is calculated. The method provided by the invention has the advantages of high sensitivity, high specificity, accuracy, simple pretreatment process, short time and greatly saved time cost.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection. Specifically, the invention relates to a method for measuring folic acid in blood spots by high-performance liquid chromatography-tandem mass spectrometry. Background technique [0002] Folic acid, also known as vitamin B9, is a water-soluble vitamin. In fact, it is one of the complexes of vitamin B, which is equivalent to pteroyl glutamic acid. Epidemiological and experimental studies have shown that folic acid deficiency and metabolic disorders seriously affect human health and are the cause of coexistence of various diseases, such as megaloblastic anemia, leukopenia, cardiovascular disease, congenital heart disease and neural tube defects, etc. . In addition, folic acid is especially important for pregnant women, and it is a consensus that pregnant women need to supplement folic acid. If folic acid is lacking in the first 3 months of pregnancy, it can lead to fetal neural tube...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 黄泽博朱监宝官培龙于嘉屏
Owner 上海可力梅塔生物医药科技有限公司
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