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Cryopreservation method of grouper sperms

A technology of cryopreservation and cryopreservation solution, which is applied in the field of cryopreservation of grouper sperm, which can solve the problems of limited artificial breeding conditions, small proportion of male fish, and scarcity of sperm, so as to achieve stable raw material sources, reasonable combination of ingredients, and vitality high effect

Inactive Publication Date: 2019-08-30
HAINAN CHENHAI AQUATIC CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although the breeding of grouper artificial breeding has been successful, its scale is still limited to a certain extent, and the conditions of artificial breeding also have natural or environmental restrictions. Whether it is fishing in natural sea areas or artificially breeding grouper, The proportion of male fish is relatively small, and only a small amount can be used as male broodstock for artificial reproduction. During the breeding process, there is often a shortage of sperm

Method used

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  • Cryopreservation method of grouper sperms
  • Cryopreservation method of grouper sperms

Examples

Experimental program
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Effect test

Embodiment 1

[0022] A grouper sperm cryopreservation solution, comprising the following raw materials in parts by weight: 32 parts of NaCl, 28 parts of KCl, CaCl 2 21 parts, trehalose 20 parts, liquid nitrogen 18 parts, antifreeze solution 20 parts, compound antibiotics 26 parts;

[0023] The antifreeze liquid is composed of methanol: glycerin: isopropyl acetate: egg yolk: honey: milk with a mass ratio of 1:1:1.3:2.1:1.5:2;

[0024] The compound antibiotic is composed of neomycin sulfate, fosfomycin and aureomycin with a mass ratio of 85:6:1;

[0025] The pH of the preservation solution is 5, and the osmotic pressure is 300mOsm / L.

[0026] Further, a method for cryopreservation of grouper sperm comprises the following steps:

[0027] S1. Sperm collection: Anesthetize the mature male grouper, dry the fish body, avoid the contact of sperm with sea water, and gently press the abdomen to collect semen;

[0028] S2. Dilution: Dilute distilled water and preservation solution with a mass ratio...

Embodiment 2

[0032] A grouper sperm cryopreservation solution, comprising the following raw materials in parts by weight: 52 parts of NaCl, 57 parts of KCl, CaCl 2 40 parts, 61 parts of trehalose, 44 parts of liquid nitrogen, 68 parts of antifreeze, 50 parts of compound antibiotics;

[0033] The antifreeze liquid is composed of methanol: glycerin: isopropyl acetate: egg yolk: honey: milk with a mass ratio of 3:3:6:3:3.2:5;

[0034] The composite antibiotic is composed of neomycin sulfate, fosfomycin and aureomycin with a mass ratio of 95:10:5;

[0035] The pH of the preservation solution is 7, and the osmotic pressure is 800mOsm / L.

[0036] A method for cryopreservation of grouper sperm comprises the following steps:

[0037] S1. Sperm collection: Anesthetize the mature male grouper, dry the fish body, avoid the contact of sperm with sea water, and gently press the abdomen to collect semen;

[0038] S2. Dilution: dilute the distilled water preservation solution with a mass ratio of 800:...

Embodiment 3

[0042] A grouper sperm cryopreservation solution, comprising the following raw materials in parts by weight: 42 parts of NaCl, 35 parts of KCl, CaCl 2 30 parts, 41 parts of trehalose, 32 parts of liquid nitrogen, 44 parts of antifreeze, 38 parts of compound antibiotics;

[0043] The antifreeze liquid is composed of methanol: glycerin: isopropyl acetate: egg yolk: honey: milk with a mass ratio of 2:2:3.8:2.4:2.0:3.1;

[0044] The compound antibiotic is composed of neomycin sulfate, fosfomycin and aureomycin with a mass ratio of 90:8:3;

[0045] The pH of the preservation solution is 6, and the osmotic pressure is 500mOsm / L.

[0046] A method for cryopreservation of grouper sperm comprises the following steps:

[0047] S1. Sperm collection: Anesthetize the mature male grouper, dry the fish body, avoid the contact of sperm with sea water, and gently press the abdomen to collect semen;

[0048] S2. Dilution: Dilute the distilled water and preservation solution with a mass ratio...

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Abstract

The invention provides a cryopreservation method of grouper sperms, and belongs to the technical field of sperm preservation. The cryopreservation method comprises raw materials in parts by weight asfollows: 32-52 parts of NaCl, 28-57 parts of KCl, 21-40 parts of CaCl2, 20-61 parts of trehalose, 18-44 parts of liquid nitrogen, 20-68 parts of an anti-freezing liquid and 26-50 parts of compound antibiotics. By combination with the cryopreservation method comprising steps of sperm collection, dilution, precooling and quick-freezing for low-temperature storage of a seminal fluid, the reactivatedsperms can keep vigor of fresh sperms by the aid of a reasonable cooling rate, and the cryopreservation method has great significance for establishment of a marine fish sperm bank and protection for germplasm resources and biodiversity.

Description

technical field [0001] The invention relates to the technical field of sperm preservation, in particular to a cryopreservation method for grouper sperm. Background technique [0002] Grouper, belonging to the order Perciformes, has a long oval body and slightly flattened sides. The mouth is large, with an auxiliary maxilla, and the teeth are fine and pointed, and some expand into canine teeth. The body is covered with small ctenoid scales, sometimes buried under the skin. The dorsal and anal fins have developed spines, the caudal fin is round or concave, and the body color varies a lot, often brown or red, with stripes and spots. It is a large and medium-sized marine fish in warm water. At present, although the breeding of grouper artificial breeding has been successful, its scale is still limited to a certain extent, and the conditions of artificial breeding also have natural or environmental restrictions. Whether it is fishing in natural sea areas or artificially breedin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0221A01N1/0226
Inventor 李伟峰陈猛猛蔡建顺蔡春有蔡有森黄春仁蔡镇辉蔡四川蔡惠明蔡金泉
Owner HAINAN CHENHAI AQUATIC CO LTD
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