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Application of phenylmethylsulfonyl fluoride (PMSF) in preparation of drugs for preventing and treating drug-induced liver injury

A drug-induced liver injury, phenylmethylsulfonyl fluoride technology, applied in the field of medicine, can solve the problem of high cost of liver transplantation

Inactive Publication Date: 2019-08-30
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Liver transplantation is not only expensive, but patients also require long-term anti-rejection drugs and ongoing follow-up treatment

Method used

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  • Application of phenylmethylsulfonyl fluoride (PMSF) in preparation of drugs for preventing and treating drug-induced liver injury
  • Application of phenylmethylsulfonyl fluoride (PMSF) in preparation of drugs for preventing and treating drug-induced liver injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0026] PMSF Intervention Animal Model Establishment

[0027] (1) Acetaminophen (Acetaminophen, APAP), purity ≥ 99.0%, purchased from Sigma-Aldrich Company. A solution of APAP at a concentration of 15 mg / ml was prepared. Weigh 0.6g of APAP powder, dissolve in 40ml of normal saline, dissolve completely in a constant temperature water bath at 60°C, and cool to 37°C for use.

[0028] (2) PMSF, with a purity of 98.5%, was purchased from Sigma-Aldrich Company. A PMSF solution with a concentration of 5 mg / ml was prepared. Weigh 50mg of PMSF, dissolve in 500μL DMSO, mix well, dissolve in 10ml normal saline, mix well again and use.

[0029] (3) Animal model establishment: 18-22g male C57BL / 6 mice were purchased from Jinan Pengyue Experimental Animal Breeding Co., Ltd. All mice were fed in an SPF animal room with a temperature of 22±2°C and a relative humidity of 50%±10%, and they had free access to food and water. 40 mice were selected, and after 3 days of adaptive feeding, they w...

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Abstract

The invention provides application of phenylmethylsulfonyl fluoride (PMSF) in preparation of drugs for preventing and treating drug-induced liver injury. An acetaminophen (APAP) liver injury animal model is established by use of APAP contaminated C57 mice, an intervention test is conducted by use of a serine protease inhibitor PMSF, and it is discovered that PMSF can effectively prevent and treatAPAP induced acute liver injury. The discovery contributes to development of prevention and control measures for APAP poisonous liver injury, and particularly has especially great significance for patients with course of diseases at a late injury stage.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of phenylmethylsulfonyl fluoride in the preparation of medicines for preventing and treating drug-induced liver injury. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Acetaminophen (APAP), also known as paracetamol, is a commonly used antipyretic and analgesic drug in clinical practice. It is also the main component of many compound preparations. Common cold medicines and painkillers contain APAP. The recommended oral dosage of APAP is 325-650mg per adult, with a maximum of 4g per day. Although APAP is safe and effective when taken within therapeutic doses, one overdo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/255A61P1/16
CPCA61K31/255A61P1/16
Inventor 宋福永李林林宋铭雪
Owner SHANDONG UNIV
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