Nondestructive diagnosis and evaluation method for intestinal health conditions of captive moschus berezovskii

A technology for intestinal health and condition, applied in disease diagnosis, material analysis by observing the impact on chemical indicators, material inspection products, etc., can solve problems such as insufficient early diagnosis technology and method

Inactive Publication Date: 2019-09-10
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Early diagnosis is the prerequisite for effective prevention and treatment of diseases. Currently, the research on intestinal d

Method used

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  • Nondestructive diagnosis and evaluation method for intestinal health conditions of captive moschus berezovskii
  • Nondestructive diagnosis and evaluation method for intestinal health conditions of captive moschus berezovskii
  • Nondestructive diagnosis and evaluation method for intestinal health conditions of captive moschus berezovskii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1. Establishment of a non-invasive diagnosis and evaluation method for the intestinal health of forest musk deer in captivity

[0051] 1. Materials and methods

[0052] 1. Selection of experimental animals and collection of feces samples

[0053] From July to August 2016, a total of 79 musk deer were selected from the Pien Tze Huang and Dajikang musk deer farms in Fengxian County, Shaanxi Province, aged 3-7 years. The feces of the test individuals were collected between 6 and 8 o'clock every morning, and about 10 g of fresh feces samples were put into a ziplock bag, marked with the sample number and collection date, sealed after the air in the bag was exhausted, and then immediately stored in a -20°C refrigerator for later use.

[0054] 2. Traditional methods to detect the intestinal health of forest musk deer

[0055] Observation of general properties of feces: Within 2 hours after the collection of feces samples, observe the appearance (including shape, colo...

Embodiment 2

[0144] Example 2. Practical application of the non-invasive diagnosis and evaluation method for the intestinal health status of captive forest musk deer

[0145] 1. Diagnosis results of digestive tract function health in 74 captive forest musk deer

[0146]According to the evaluation system process established in Example 1, the intestinal health status of 74 experimental individuals was evaluated and diagnosed. A preliminary screening of the immune function of the digestive tract showed that the IgA content of 10 individuals was lower than the normal range, and the ear numbers were B3, B2, B8, B9, A7, A3, A8, D6, D2 and D5; the IgA content of 48 individuals Normal; 16 IgA levels were higher than the normal range (Table 12). The results of the preliminary evaluation of gastrointestinal immune function, gastrointestinal bleeding and stool appearance showed that there were 16 individuals with abnormalities, of which 5 individuals were initially judged as inflammatory bowel disea...

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Abstract

The invention discloses a nondestructive diagnosis and evaluation method for intestinal health conditions of captive moschus berezovskii, and provides an application of a substance for detecting the fecal appearance and fecal occult blood of the moschus berezovskii and the contents of immunoglobulin A, calprotectin and lactoferrin in the feces to the preparation of products for assessing the intestinal health conditions and/or intestinal disease types of the moschus berezovskii. According to the method, the fecal appearance, fecal occult blood and contents of immunoglobulin (such as IgA, lactoferrin and calprotectin) of the moschus berezovskii with healthy feces and abnormal feces are respectively measured, reference constants of physiological indexes such as fecal occult blood and immunoglobulin of feces of the captive moschus berezovskii are explored, and certain basis is provided for the clinic diagnosis of the intestinal health conditions and intestinal inflammation diseases of thecaptive moschus berezovskii.

Description

technical field [0001] The invention relates to a non-injury diagnosis and evaluation method for the intestinal health status of captive forest musk deer in the field of biotechnology. Background technique [0002] The breeding of forest musk deer (Moschus berezovskii) is an important way to alleviate the pressure on wild musk deer resources and ensure the sustainable utilization of musk deer resources. It is also the provenance basis for reintroduction and reconstruction of wild populations (Sheng & Liu, 2007; He et al. ., 2003; Wang et al., 2016). The artificial breeding of forest musk deer in my country began in 1958, and has made remarkable progress in many aspects after years of exploration (Xue et al., 2008; Liu et al., 2010). However, the breeding population has been plagued by unstable health and high incidence of diseases for a long time, which seriously restricts the development of the population (Xu et al., 2003; He et al., 2014; Wei et al., 2016). The domestica...

Claims

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Application Information

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IPC IPC(8): G01N33/483G01N21/78G01N33/68
CPCG01N21/78G01N33/483G01N33/6854G01N33/6893G01N2333/79G01N2800/065
Inventor 周俊彤孙晓宁徐尚华胡德夫
Owner BEIJING FORESTRY UNIVERSITY
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