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Application of galanthamine in prevention and treatment for fish enteritis caused by soybean meal feed

A galantamine, fish technology, applied in the application field of fish enteritis, to achieve the effect of inhibiting intestinal inflammation, avoiding residues and environmental safety problems, and improving growth efficiency

Active Publication Date: 2019-11-15
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding its anti-inflammatory effect, it has only been seen to have a certain effect on hyperemia.

Method used

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  • Application of galanthamine in prevention and treatment for fish enteritis caused by soybean meal feed
  • Application of galanthamine in prevention and treatment for fish enteritis caused by soybean meal feed
  • Application of galanthamine in prevention and treatment for fish enteritis caused by soybean meal feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Pharmacodynamic prediction of the functional ingredient galantamine for alleviating enteritis

[0038] The KEGG pathway analysis of the grass carp transcriptome suggests the existence of the cholinergic anti-inflammatory pathway in fish: the inventors have shown that the intestinal inflammation of grass carp in the 40% soybean meal replacement group has a self-healing effect from week 5 to week 7. ability. At each stage of the course of the disease, including 0 days, 3 weeks, 5 weeks, and 7 weeks, the transcriptome of the intestinal tissue was measured, and the analysis results in the KEGG pathway showed that the acetylcholine (Acetylcholine) pathway was involved in the occurrence and regression of soybean meal-induced enteritis. are activated ( figure 2 ), implying that the fish cholinergic anti-inflammatory pathway may exist.

[0039] Then, we used the CMap database to predict the efficacy of the cholinesterase inhibitor galantamine: Galantamine, as a cho...

Embodiment 2

[0046] Example 2 Application of galantamine as a feed additive in alleviating soybean meal-induced zebrafish enteritis

[0047] 1. Zebrafish SBMIE modeling and galantamine added experimental feed formula and preparation method

[0048] Table 3 Zebrafish SBMIE modeling and galantamine added experimental feed formula

[0049]

[0050] FM is the contrast fishmeal feed, 50SBM is the soybean meal feed (50% replacement protein source) used in this embodiment, 50SBM+0.5ppm Gal is the soybean meal feed with 0.5ppm Galantamine added, and 50SBM+1ppm Gal is the soybean meal feed with 1ppm Galantamine added sensitive soybean meal feed.

[0051] The preparation method is as follows:

[0052] (1) Pass fishmeal, soybean meal, starch, flour, cellulose, mineral premix, vitamin premix, etc. through a 60-mesh sieve, among which there may be larger particles such as fish bones and soybeans in the fish meal, soybean meal, and mineral mixture. Leather, etc., should be crushed and passed throu...

Embodiment 3

[0071] Example 3 Intramuscular injection of galantamine reduces the expression of intestinal inflammatory factors in diseased grass carp after soybean meal-induced enteritis

[0072] 1. Grass carp fed with 70% soybean meal feed group and fish meal group for 7 weeks (see Integrative Transcriptomic Study Reveals Immune Mechanism for the Resilience to Soybean Meal Stress in Fish Gut and Liver, Frontier in Physiology for grass carp SBMIE modeling method and feed formula, etc.) 10 tails, keep the temperature in the cage at 23-25°C, the dissolved oxygen is greater than 5mg / L, fasting;

[0073] 2. Galantamine hydrobromide (0.05-0.1 mg / kg fish body weight) was injected intramuscularly into the back, twice a day for a total of 2 days, and intestinal tissue samples were taken on the 4th day;

[0074] 3. Perform fluorescent quantitative qPCR analysis to detect the expression of immune genes related to intestinal inflammation. The results of statistical analysis of fluorescence quantitat...

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Abstract

The invention discloses application of galanthamine in prevention and treatment for soybean meal-induced enteritis of fishes. According to a neuroregulation theory of intestinal mucosal immunity, in combination with two methods of disease modeling and biological information analysis, it is inferred that the galanthamine is a potential medicine for preventing and treating the soybean meal-induced enteritis; it is proved by an experiment that the galanthamine can activate expression of anti-inflammatory cytokines in intestinal tracts of bred fishes through addition into feed or intramuscular injection in backs, inhibit intestinal inflammations, maintain tight junctions of intestinal epithelium barriers, and inhibit intestinal epithelium apoptosis, therefore the enteritis caused by a soybeanmeal feed is relieved, recovery of growth of the fishes can be achieved, and therefore the galanthamine can be taken as a novel feed additive with an anti-inflammatory effect or an injection with an anti-inflammatory effect for injection operation.

Description

technical field [0001] The invention belongs to the field of veterinary medicine, and in particular relates to the application of galantamine in preventing and treating fish enteritis induced by soybean meal. Background technique [0002] Aquatic animal enteritis is one of the main diseases that cause economic losses in aquaculture. Aquatic animals, especially fish and shrimp, need to add animal protein such as fishmeal to their feed. However, the shortage of fishmeal resources has become a key factor limiting the sustainable development of my country's aquaculture industry. Therefore, plant protein sources are used to replace fishmeal and improve its quality. Utilization rate is an inevitable choice for the development of aquaculture industry. However, plant protein sources, such as soybean meal, cotton meal, etc., generally induce intestinal inflammation, liver disease and growth decline in fish when the proportion of fish meal is too large, which limits the application of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/37A23K20/132A61K31/55A61P29/00A61P1/00
CPCA23K50/80A23K10/37A23K20/132A61K31/55A61P29/00A61P1/00Y02P60/87
Inventor 张永安吴南王标
Owner INST OF AQUATIC LIFE ACAD SINICA
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