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Preparation method of polyphenol-amyloid fiber hydrogel for relieving acute intestinal inflammation of mice

A technology for intestinal inflammation and amyloid, which is applied in the field of preparation of polyphenol-amyloid fiber hydrogel, can solve problems such as incurable and affecting kidney function, and achieve the effect of increasing the loading capacity

Inactive Publication Date: 2020-12-22
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

IBD produces symptoms including weight loss, bloody diarrhea, and severe abdominal pain. There is currently no cure, and the most commonly used drugs such as aminosalicylates and immunomodulators have shown serious side effects, such as affecting kidney function.

Method used

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  • Preparation method of polyphenol-amyloid fiber hydrogel for relieving acute intestinal inflammation of mice
  • Preparation method of polyphenol-amyloid fiber hydrogel for relieving acute intestinal inflammation of mice
  • Preparation method of polyphenol-amyloid fiber hydrogel for relieving acute intestinal inflammation of mice

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Egg white lysozyme was purified by dialysis with ultrapure water at 4° C., and then the lysozyme solution after dialysis was subjected to freeze-drying treatment. Weigh a certain quality of egg white lysozyme freeze-dried powder and dissolve it in ultrapure water to prepare a 2wt% egg white lysozyme solution, adjust the pH to 2 with HCl, and incubate with magnetic stirring at 90°C for 8 hours to obtain egg white Lysozyme Amyloid Fiber Mother Solution.

[0017] (2) Weigh a certain amount of EGCG in Bis-Tris buffer (10mM, pH 7.2), mix the egg white lysozyme amyloid fiber and EGCG solution obtained in step (1) with a certain volume ratio, and stir magnetically for 30s. The polyphenol-amyloid fiber hydrogel (EGCG concentration: 25 mg / mL) was formed after standing still.

[0018] (3) Thirty C57BL / 6J male mice were randomly divided into normal diet control group (NC), DSS induction group and DSS induction + hydrogel group, 10 mice in each group. After one week of adapti...

Embodiment 2

[0020] (1) Detection of inflammatory factors in serum

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Abstract

The invention relates to a preparation method of polyphenol-amyloid fiber hydrogel for relieving acute intestinal inflammation of mice. Egg white lysozyme amyloid fiber is prepared through heating under a high-temperature acidic condition, polyphenol is simply added into the amyloid fiber, and reversible hydrogel is formed through self-assembly. By constructing an IBD animal model, the hydrogel isused for gavage treatment of the mice, and the inventor finds that the hydrogel can significantly relieve DSS-induced acute colitis. All the materials used in the invention are food-grade and are safer than medicines for preventing or treating intestinal inflammation, and a new thought is provided for developing tea polyphenol products and medicines having anti-inflammatory activity and improvedbioavailability.

Description

technical field [0001] The invention relates to the technical field of preparing a polyphenol-amyloid fiber hydrogel and alleviating DSS-induced acute intestinal inflammation in mice. Background technique [0002] Inflammatory bowel disease (inflammatory bowel disease, IBD) is a chronic non-specific inflammatory disease, including ulcerative colitis (ulcerative colitis, UC) and Crohn's disease (Crohn's disease, CD). IBD produces symptoms including weight loss, bloody diarrhea, and severe abdominal pain. There is currently no cure, and the most commonly used drugs such as aminosalicylates and immunomodulators have shown serious side effects, such as affecting kidney function. Dietary polyphenols in foods and plants have attracted widespread interest in the prevention and treatment of colitis in recent years, and many dietary polyphenols have been used to prevent and treat chronic and acute colitis in rodent models. Polyphenolic compounds have been shown to be able to induce ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K31/353A61K47/36A61P1/00A61P29/00
CPCA61K9/06A61K31/353A61K47/36A61P1/00A61P29/00
Inventor 胡冰于诗婕
Owner NANJING AGRICULTURAL UNIVERSITY
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