Method and device for evaluating immunity level

A technology of immunity and level, applied in the field of immunity detection, can solve the problems of lack of comprehensiveness, sensitivity and accuracy, and rough assessment

Active Publication Date: 2019-09-17
CHENGDU EXAB BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It overcomes the problem that the existing technology can only target a certain aspect of the immune system, and can only conduct a relatively rough assessment, and lacks comprehensiveness, sensitivity and accuracy

Method used

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  • Method and device for evaluating immunity level
  • Method and device for evaluating immunity level
  • Method and device for evaluating immunity level

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 The establishment of immunographic analysis and immune level evaluation method

[0049] 1. Lymphocyte isolation and mRNA extraction

[0050] 1. Lymphocyte isolation

[0051] Take 10ml of venous blood from 6 subjects, put it in a purple-capped EDTA anticoagulant tube, and use human lymphocyte separation medium (Tianjin Haoyang, product number LTS1077) to separate lymphocytes. The specific operation steps are as follows:

[0052] (1) Add 10ml of collected fresh blood into a 50ml centrifuge tube, add physiological saline to make the volume to 25ml, and gently blow and mix.

[0053] (2) In a new 50ml centrifuge tube, add 25ml of lymphocyte separation medium (equal volume), then use a pipette to draw the blood sample and gently add it on the surface of the separation medium along the wall, centrifuge at 500g, 20°C 30min, the rising speed is 2, and the falling speed is 1 (Thermol low-speed centrifuge).

[0054] (3) After centrifugation, use a 10ml pipette to care...

Embodiment 2

[0161] Embodiment 2 The method of the present invention evaluates the immune level of a large number of subjects

[0162] According to the method described in Example 1, 124 subjects were subjected to immune atlas detection and analysis, and all subjects corresponded to D 50 The values ​​are as follows:

[0163]

[0164]

[0165]

[0166]

[0167] Among them, healthy people:

[0168]

[0169]

[0170]

[0171] Leukemia patients:

[0172]

[0173]

[0174] cancer patient:

[0175] Numbering D 50

cases 24ny 0.04 liver cancer 1ny 0.049 lung cancer 2ny 0.021 lung cancer 3ny 0.0196 lung cancer 4ny 0.006 lung cancer 5ny 0.021 lung cancer 7ny 0.001 lung cancer 9ny 0.042 lung cancer 12-1ny 0.004 colon cancer 13-1ny 0.093 stomach cancer 65-1 0.011 breast cancer

[0176] Ideally, each CDR3 clone has only one functional copy, D 50 =0.5; when the subject'...

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Abstract

The invention discloses a method and a device for evaluating an immunity level. The method comprises the steps of separating lymphocyte from a testee sample, extracting the RNA of the lymphocyte and performing reverse transcription, obtaining a BCR and / or TCR variable region polynucleotides by means of a specific primer, performing sequencing on the polynucleotides for obtaining sequence information, and calculating the D50 value of a BCR and / or TCR variable region sequence, thereby determining the immunity level of the testee.

Description

technical field [0001] The invention relates to the field of immunity detection, in particular to a method for evaluating immunity level through immune map analysis. Background technique [0002] The main methods currently on the market for analyzing immune function are: 1) five items of immunity, detecting the content of immunoglobulin and complement in the blood; 2) blood routine, using cell counting methods to analyze the number of white blood cells in peripheral blood 3) analysis of lymphocyte subsets, using flow cytometry analysis and PCR technology to analyze the number and relative proportion of each subset of leukocytes in peripheral blood. [0003] five immune tests [0004] Immunoglobulin G (IgG) and immunoglobulin A ( IgA), immunoglobulin M (IgM), complement C3 and C4 content. Immunoglobulin and complement are the main effect components of humoral immunity. In the case of certain diseases (such as infection, autoimmune disease, immunodeficiency disease, etc.), ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B15/20G16B20/20G16B30/10G16B35/10
CPCG16B15/20G16B20/20G16B30/10G16B35/10
Inventor 张志新卓越侯百东华兆琳叶树剑
Owner CHENGDU EXAB BIOTECH CO LTD
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