A kind of integrated nanomaterial for diagnosis and treatment and its preparation method and application

A technology of nanomaterials and reactions, applied in the direction of nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of the inability to determine the enrichment of photothermal therapeutic agents in tumor tissue locations, detection of therapeutic effects, application limitations, etc., and achieve photothermal conversion High efficiency, good biocompatibility, and high relaxation efficiency

Active Publication Date: 2021-08-03
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Photothermal therapy requires the entire tumor tissue to be effectively exposed to light, but it is impossible to determine the location and size of the tumor tissue, the enrichment of photothermal therapeutic agents in it, and the detection of therapeutic effects, which limits the application of PTT.

Method used

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  • A kind of integrated nanomaterial for diagnosis and treatment and its preparation method and application
  • A kind of integrated nanomaterial for diagnosis and treatment and its preparation method and application
  • A kind of integrated nanomaterial for diagnosis and treatment and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Preparation of nanomaterials

[0036] (1) Add 100mg DL-dopa and 100mg sericin to 100mL deionized water, mix well to make a mixed solution; add 20mg KMnO to 5mL deionized water 4 KMnO 4 solution; the KMnO 4 The solution is slowly added dropwise to the mixed solution, and the dropwise addition process is about 1-2 minutes. After the dropwise addition is completed, the reaction is continued, and the reaction time is 1-4 hours.

[0037] (2) Dialysis (molecular weight cut-off Mw=3500Da) for 24-48 hours.

[0038] (3) Concentrate by ultrafiltration and centrifugation to obtain nanomaterials with corresponding concentrations.

[0039] 2. Performance analysis of nanomaterials

[0040] Such as figure 1 As shown, 200 μL of the nanomaterial was taken in a cuvette, and the average particle diameter of the nanomaterial was measured by a dynamic light scattering instrument to be 100 nm, and the distribution coefficient PDI was 0.026.

[0041] Such as figure 2 As shown, take...

Embodiment 2

[0051] Nanomaterials were prepared according to the reaction conditions in Table 1, and the process flow was referred to in Example 1. The particle size of the nanomaterials prepared in Table 1 was shown.

[0052] Table 1

[0053]

[0054] It can be seen from Table 1 that when DL-dopa, sericin and KMnO 4 When the mass ratio is 5:5:1, the particle size of the obtained nanomaterials is the best. When KMnO 4 The concentration of nanomaterials is 30mg / 105mL. The particle size distribution of nanomaterials is too wide and too large. Too large nanoparticles cannot be enriched near the tumor tissue through the EPR effect, and are easily trapped by the reticuloendothelial system, which makes the effects of contrast and photothermal therapy less effective. decline.

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Abstract

The invention discloses a diagnosis and treatment integrated nanometer material and its preparation method and application. The preparation method comprises: adding dopa, sericin and KMnO in water 4 , reaction polymerization to form the nanomaterial. The present invention self-assembles through two oxidation reactions to form a high-efficiency nano-contrast agent, which has the advantages of high relaxation efficiency and good biocompatibility; The photothermal therapeutic agent is used in photothermal therapy, and at the same time, the position and size of the tumor tissue and the enrichment of the photothermal therapeutic agent in the tumor tissue can be monitored by magnetic resonance technology, so as to evaluate the therapeutic effect.

Description

technical field [0001] The invention relates to the field of integrated diagnosis and treatment of photothermal therapy mediated by magnetic resonance imaging, in particular to a nanomaterial for integrated diagnosis and treatment, its preparation method and application. Background technique [0002] Photothermal therapy (PTT) is a new type of tumor treatment method, which uses photothermal therapeutic agents to absorb near-infrared light, convert light energy into heat energy, and increase the temperature of tumor tissue, thereby inducing cell apoptosis and achieving the purpose of tumor suppression . Photothermal therapeutic agents are materials with high photothermal conversion efficiency. Gold nanoparticles, carbon nanomaterials, copper sulfide nanoparticles and some organic nanoparticles can be used as photothermal therapeutic agents for tumor photothermal therapy. In order to avoid the influence of blood, water and protein in the tissue, reduce tissue penetration, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/18A61K41/00A61P35/00B82Y5/00B82Y15/00
CPCA61K41/0052A61K49/1818A61P35/00B82Y5/00B82Y15/00
Inventor 唐建斌田丹肖冰申有青
Owner ZHEJIANG UNIV
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