A manganese-based integrated nanomaterial for tumor diagnosis and treatment, preparation method and application

A nanomaterial, tumor technology, applied in antitumor drugs, photodynamic therapy, preparations for in vivo experiments, etc., can solve the problems of lack of targeting, short circulation time in vivo, low R1, etc., and achieve high biocompatibility , The effect of long circulation time in the body and small toxic and side effects

Active Publication Date: 2021-12-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing small molecule CAs have disadvantages such as low R1, short circulation time in vivo, and lack of targeting.

Method used

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  • A manganese-based integrated nanomaterial for tumor diagnosis and treatment, preparation method and application
  • A manganese-based integrated nanomaterial for tumor diagnosis and treatment, preparation method and application
  • A manganese-based integrated nanomaterial for tumor diagnosis and treatment, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1, preparation of nanomaterials

[0034] (1) Golden tonnin (HYP) (0.61 mg) is dissolved in 200 μl dimethyl sulfoxide (DMSO). The manganese chloride (1.2 mg) is called in 200 μl of deionized water. Bao serum albumin (BSA) (400 mg) is dissolved in 5.0 ml of deionized water, add 1 M NaOH to the BSA solution to adjust the pH to 10, and then HYP and MNCl 2 The solution was slowly added to the BSA solution in turn, followed by tapping the pH of the mixture to 9.

[0035] (2) At room temperature, the expansion reaction was 2 hours.

[0036] (3) The dialysis film (3500 Da) was dialyzed in deionized water for 24 hours.

[0037] (4) lyophilized to obtain brown powdered BHM nanoparticles.

[0038] 2, performance analysis of nanomaterials

[0039] (1) The above BHM nanoparticles are detected using a dynamic light scatterometer (DLS). figure 1 As shown, the dynamic light scattering granularizer (DLS) determines that the average particle diameter of BHM nanoparticles is 67 nm, and the pa...

Embodiment 2

[0049] The nanomaterials were prepared according to the reaction conditions of Table 1, and the process flow referred to in the particle diameter of the nanomaterial obtained in Example 1 is shown in Table 1.

[0050] Table 1

[0051]

[0052] It can be seen from Table 1 that when bovine serum albumin, the mass ratio of golden hormone and chloride is 400: 0.61: 1.2, the particle diameter of the nanomaterial is best. When the concentration of manganese chloride is increasing, the particle size distribution of nanomaterials is increasing, and excessive nanoparticles cannot be enriched in the vicinity of tumor tissue by the EPR effect, and is easily intercepted by a mesh endothelium. And the effect of photodynamic therapy is lowered.

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Abstract

The invention discloses a manganese-based integrated nanometer material for tumor diagnosis and treatment, a preparation method and an application. In the preparation method, albumin, hypericin and manganese chloride are added into the water medium, and the products are self-assembled to form the integrated nanometer material for tumor diagnosis and treatment. The invention prepares a high-efficiency manganese-based nano-scale macromolecular contrast agent through self-assembly, and has the advantages of high relaxation efficiency, long circulation time in the body, rapid renal clearance, targeting, high biocompatibility, and small toxic and side effects. The nanomaterials prepared by the present invention have higher photodynamic conversion efficiency at 595nm, and can be used as photosensitizers in photodynamic therapy. In addition, the position and size of tumors and the enrichment of phototherapeutic agents in tumor tissues can be monitored by means of magnetic resonance technology. , used to evaluate the therapeutic effect and realize the integration of diagnosis and treatment of photodynamic therapy mediated by magnetic resonance imaging.

Description

technical field [0001] The invention relates to the field of integrated materials for diagnosis and treatment of photodynamic therapy mediated by magnetic resonance imaging, in particular to a manganese-based nanometer material for integration of diagnosis and treatment of tumors, a preparation method and an application. Background technique [0002] Photodynamic therapy (PDT) is a non-invasive, light-excited cancer treatment modality. The elements involved in PDT treatment are excitation light, photosensitizer and molecular oxygen. Under the excitation of a light source with a specific wavelength, photosensitizers can be selectively activated to generate ROS, followed by cytotoxicity, vascular damage and immune regulation, and finally induce cancer cell death. Photosensitizers are a necessary part of PDT. The common ones are porphyrin-related macrocyclic compounds, non-porphyrin macrocyclic compounds (such as phthalocyanine, porphine, hypericin and phenothiazines, etc.), a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/18A61K49/14A61K41/00A61P35/00
CPCA61K49/1869A61K49/143A61K41/0057A61P35/00
Inventor 胡红杰周晓璇唐建斌韩玉鑫
Owner ZHEJIANG UNIV
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