Application of DDX20 in preparation of rectal cancer postoperative prognosis evaluation kit

A DDX20, 1. DDX20 technology is applied in the application field of DDX20 in the preparation of rectal cancer postoperative prognosis evaluation kits, which can solve the problems of lack of specific molecular targets and unclear molecular mechanisms for treatment.

Inactive Publication Date: 2019-09-27
FUJIAN NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Rectal cancer is one of the most threatening tumors to human health. The molecular mechanism of its occurrence is still unclear, and its treatment also lacks specific molecular t

Method used

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  • Application of DDX20 in preparation of rectal cancer postoperative prognosis evaluation kit
  • Application of DDX20 in preparation of rectal cancer postoperative prognosis evaluation kit
  • Application of DDX20 in preparation of rectal cancer postoperative prognosis evaluation kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (a) Pathological specimens were obtained from biopsies or intraoperative and postoperative pathological materials of rectal cancer patients.

[0042] (b) The immunohistochemical method uses the SP staining method, and the specific steps are as follows:

[0043] (c) Paraffin sections of rectal cancer tissues were prepared, and the oven was set at 60° C. overnight.

[0044] (d) Dewaxing of slices. Soak in sequence: Xylene I: 10min; Xylene II: 10min; Xylene III: 10min.

[0045] (e) Section hydration. Soak in sequence: absolute ethanol: 3 min; 90% (v / v) ethanol: 3 min; 80% ethanol: 3 min; 75% ethanol: 3 min.

[0046] (f) Washing with PBS 3 times, 5 min each time.

[0047](h) EDTA antigen high-pressure repair: soak the slices in 0.01M EDTA repair solution, soak in boiling water for 5 minutes, and cool to room temperature. Wash with PBS 3 times, 5min each time.

[0048] (I) Add 300 μL of 3% (w / w) hydrogen peroxide aqueous solution, 37° C. for 10 min. Wash with PBS 3 ti...

Embodiment 2

[0063] A postoperative tumor sample of rectal cancer was taken for paraffin-embedded section, and detected by the above-mentioned immunohistochemical method. After calculation, the DDX20 immunohistochemical score of the cancer tissue was 0.621. After postoperative follow-up, it was found that the patient had recurrence and metastasis of rectal cancer in the 35th month after the operation, and died 46 months after the operation.

Embodiment 3

[0065] A postoperative tumor sample of rectal cancer was taken for paraffin-embedded section, and the above-mentioned immunohistochemical method was used for detection. After calculation, the DDX20 immunohistochemical score of the cancer tissue was 0.399. After postoperative follow-up, it was found that the patient was alive without metastasis and recurrence 5 years after the operation.

[0066] From the above test results, it can be known that detecting the relative expression of DDX20 molecules by immunohistochemical method can predict the risk of distant metastasis of rectal cancer and the postoperative survival or death of patients. When the DDX20 immunohistochemical score of cancer tissue is higher than 0.532, rectal cancer is prone to recurrence and metastasis, and rectal cancer patients are likely to die after surgery. Obviously, DDX20 is related to rectal cancer. Therefore, using DDX20 as a protein molecular marker to detect its expression level can predict recurrence ...

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Abstract

The invention provides a new application of DDX20 in preparation of a rectal cancer postoperative prognosis evaluation kit. The inventor discovers for the first time that the risk of rectal cancer recurrence and metastasis of a rectal cancer patient can be determined by detecting the relative expression quantity of DDX20 in rectal cancer tissues by adopting an immunohistochemical method. The application of DDX20 in preparation of the rectal cancer postoperative prognosis evaluation kit indicates that the protein can be used for preparing a protein molecular marker for determining the prognosis of the rectal cancer patient and has the important guiding significance for postoperative monitoring and sequential treatment of the rectal cancer patient.

Description

technical field [0001] The invention relates to the application of DDX20 in the preparation of a kit for evaluating prognosis after operation of rectal cancer. Background technique [0002] Rectal cancer refers to cancer between the dentate line and the rectosigmoid junction, and is one of the most common malignant tumors of the digestive tract. The low position of rectal cancer is easy to be diagnosed by digital rectal examination and sigmoidoscopy. However, because of its location deep into the pelvic cavity, the anatomical relationship is complicated, the operation is not easy to complete, and the postoperative recurrence rate is high. The middle and lower rectal cancer is close to the anal sphincter. It is difficult to preserve the anus and its function during surgery. The median age of onset of rectal cancer in my country is about 45 years old. The incidence rate among young people tends to increase. [0003] DDX20 is a member of the ATP-dependent RNA helicase famil...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/57446G01N33/57484G01N33/6854G01N33/6893G01N2800/50G01N2800/52G01N2800/54
Inventor 林尧王清水
Owner FUJIAN NORMAL UNIV
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