A method for the detection of nucleic acid molecules based on cyclic amplification and cation exchange

Abstract

The invention belongs to the technical field of nucleic acid molecular detection, and discloses a method for detecting nucleic acid molecules based on cyclic amplification and cation exchange, using a 3D nano-walker to identify and amplify miRNA141, combined with magnetic beads and cleavage enzyme cycle technology to release Silver nanoparticles convert nanoparticles into ions by acid, and exchange cations with CdSe, a photoelectric material, to reduce the photoelectric signal of CdSe and realize the detection of miRNA141. Under the action of light, the material with photoelectrochemical activity in the present invention, the outer layer electrons absorb energy to generate a transition to transfer charge, and the electrode material is modified; it can be combined with DNA, antibody, nano gold and other substances, which greatly enriches the photoelectrochemistry The application range of the sensor can also be combined with PCR, enzyme-linked reaction, cycle amplification and other methods to improve the sensitivity of detection.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid molecule detection, and in particular relates to a method for detecting nucleic acid molecules based on cycle amplification and cation exchange. Background technique [0002] At present, the closest existing technology: at present, the detection of miRNA is closely related to the occurrence of tumors, so it serves as an important basis for early diagnosis of tumors. Existing fluorescence, aggregation amplification and imprinting techniques are not sensitive enough for detection and have poor specificity. Photoelectric detection technology is widely used in the detection of nucleic acid molecules due to its good stability, low background signal and wide detection range. At present, the method for detecting miRNA is mainly to combine DNA molecular machines with electrochemiluminescence sensing. First, the quantum dots are brought close to the gold nanoparticles, and resonance energy transfer o...

AI Technical Summary

Problems solved by technology

[0008] (1) Current 3D DNA molecular machines use metal particles as substrates and immobilize DNA probes on their surfaces. If the probes are disorderly and disorderly arranged, there are also many non-specific adsorptions between metal particles and probes, resulting in 3D DNA nanomachines operate less efficiently

[0009] (2) The sensitivity of existing nucleic acid molecular detection is low

Images