A kind of production method of pig placenta polypeptide
A production method, pig placenta technology, applied in the field of pig placenta polypeptide production, can solve the problems of high corrosion of equipment, unfavorable industrial production, low extraction rate, long enzymatic hydrolysis time, etc.
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Embodiment 1
[0041] 1 kg of fresh pig placenta is washed and crushed to obtain minced pig placenta. Add 5kg of water to the minced meat, stir evenly, and then add 25g of compound enzyme to obtain a mixed solution. The ratio of the compound enzyme is trypsin:papain:flavor protease=1:1:2. Vacuum-pack the mixed solution into two bags, then sterilize by irradiation on one side with a radiation dose of 6 kGy, and then perform an enzymatic hydrolysis reaction at 45°C for 20 hours to obtain an enzymatic hydrolysis solution. Finally, the enzymolysis solution is filtered and then spray-dried to obtain porcine placenta polypeptide powder.
Embodiment 2
[0043] 1 kg of fresh pig placenta is washed and crushed to obtain minced pig placenta. Add 3kg of water to the minced meat, stir evenly, and then homogenize at 10000r / min for 2min to obtain a homogeneous liquid. Put the homogeneous liquid into the subcritical equipment to decompose for 60min, the subcritical condition is 130°C, 1Mpa. Add 10 g of compound enzyme to the subcritically decomposed pig placenta fluid to obtain a mixed solution, and the ratio of the compound enzyme is trypsin:papain:flavor protease=2:1:1. The mixed solution was vacuum-packed into two bags, and then sterilized by irradiation on both sides with a total irradiation dose of 6 kGy, and then enzymatically reacted at 37° C. for 8 hours to obtain an enzymolyzed solution. Finally, the enzymolysis solution is filtered and then spray-dried to obtain pig placenta powder.
Embodiment 3
[0045] 1 kg of fresh pig placenta is washed and crushed to obtain minced pig placenta. Add 10kg of water to the minced meat, stir evenly, and then homogenize at 5000r / min for 5min to obtain a homogeneous liquid. Put the homogeneous liquid into the subcritical equipment to decompose for 30min, the subcritical condition is 150℃, 0.5Mpa. Add 20 g of compound enzyme to the subcritically decomposed pig placenta fluid to obtain a mixed solution, and the ratio of the compound enzyme is trypsin:papain:flavor protease=2:1:2. The mixed solution was vacuum-packed into 4 bags, and then sterilized by double-sided irradiation with a total irradiation dose of 6 kGy, and then enzymatically reacted at 50° C. for 4 hours to obtain an enzymolyzed solution. Finally, the enzymolysis solution is filtered and then spray-dried to obtain pig placenta powder.
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