Single-layer microfluidic chip with three-dimensional focusing function

Abstract

The invention discloses a single-layer microfluidic chip with three-dimensional focusing function. The single-layer microfluidic chip comprises a single-layer microfluidic chip main body, and a main runner and a focusing module that are arranged disposed in the single-layer microfluidic chip main body. The focusing module includes a sample pipeline communicated with the main runner, a first sheathliquid pipeline and a second sheath liquid pipeline that are communicated with the main runner to realize focusing of a sample flow in the main runner in a vertical direction, and a third sheath liquid pipeline and a fourth sheath liquid pipeline that are communicated with the main runner to realize focusing of the sample flow in the main runner in a horizontal direction. By means of the sheath liquid in the first sheath liquid pipeline and the second sheath liquid pipeline, the single-layer microfluidic chip provided by the invention realizes focusing of the sample flow in the XZ plane, andthrough the third sheath liquid pipeline and the fourth sheath liquid pipeline, focusing in the XY plane can be realized, thereby realizing three-dimensional focusing. The single-layer microfluidic chip enables single-row flow of the sample in the main runner, not only improves the precision of analysis and sorting, but also has a simple structure and is low in cost.

Description

technical field [0001] The invention relates to the field of microfluidic chips, in particular to a single-layer microfluidic chip with a three-dimensional focusing function. Background technique [0002] The development of modern life science is inseparable from the innovation and progress of research methods, especially the development of separation, analysis and testing methods for micron or submicron particles, which has greatly promoted the development of modern life science to a higher, deeper and more refined level. level development. Since 1990, when scientists first proposed a miniature total analysis system, microfluidic technology has gradually become an important development frontier in the current microscopic field of analysis. It can integrate the sample preparation, reaction and other operations of biological, chemical, and medical analysis processes into a single micron scale. On the chip, the whole process of analysis is automatically completed, which is ca...

AI Technical Summary

Problems solved by technology

However, traditional microfluidic chips are processed based on planar design and have equal depths in the direction perpendicular to the substrate. Therefore, samples can only be arranged along the plane direction parallel to the substrate, but cannot be arranged in the direction perpendicular to the substrate. To achieve effective aggregation arrangement, the microfluidic chip based on this design can only achieve the so-called "one-dimensional focusing", which reduces the accuracy of analysis and sorting

[0004] In order to solve the three-dimensional focusing problem on this microfluidic chip, it is often necessary to achieve three-dimensional focusing by stacking multi-layer chips, but this method has a complex structure, high design difficulty, and high processing costs, which cannot be achieved in practical applications. good promotion

[0005] On the other hand, the outlet channel of the existing microfluidic chip generally includes at least one sorting channel and a waste liquid channel, so that a protrusion relative to the direction of fluid flow will be formed at the intersection of the two outlet channels, and a bulge will be formed in the raised area. The stagnation point in the flow, the existence of this stagnation point causes cells or particles to adhere to it, which in turn causes the aggregation of cells or particles, resulting in changes in the stable microfluidic flow state, reducing the accuracy of analysis or sorting, and also causing flow Channel congestion, sample contamination, purity drop, etc.

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