Composite biological agent for improving the survival rate of prawn culture and its application method

A biological agent and survival rate technology, applied in the field of compound biological agent preparation, can solve problems such as neglect, poor application effect, and breeding failure, and achieve the effect of reducing niche width, improving redox potential, and maintaining long-term stability

Active Publication Date: 2020-07-28
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, biological agents in the field of prawns adjust the breeding environment from the perspective of "individual microorganisms". Lead to poor application effect or even breeding failure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Intensive high-density application case of high-level ponds for prawns. This embodiment includes component A and component B, wherein component A is 50% of Paracoccus holophagous, 25% of Pseudomonas, and 25% of Marinobacter spp. Component B is 20% vanillin, 15% acetyltransferase, 15% activated carbon powder, 20% polyacrylamide, 15% sodium lauryl sulfate, and 15% calcium peroxide. The dosage of component B is 10 mg / m 3 Water body; the dosage of component A after expansion is 0.5 mL / m 3 Water body.

[0031] Table one is the effect that this embodiment is applied to prawn elevated pond culture:

[0032] Table 1. Application effect of compound preparation high pool

[0033] project value NH before administration 4 + -N (mg / L)

[0034] Remarks: a, the breeding process was 88 days, and the compound preparation was used 5 times in total, with an average interval of 15 days; b, except for the compound preparation, other operations were the same as in ...

Embodiment 2

[0036] Application case of shrimp culture in earthen ponds. This embodiment includes component A and component B, wherein component A is 32% of Paracoccus holophagous, 33% of Pseudomonas, and 35% of Marinobacter alkanes. Component B is vanillin 10%, acetyltransferase 15%, sodium nitrate 10%, activated carbon powder 10%, calcium hydroxide 5%, polyacrylamide 20%, sodium lauryl sulfate 15%, sodium percarbonate 15% %. The dosage of component B is 5mg / m 3 Water body; the dosage of component A after expansion is 0.3 mL / m 3 Water body.

[0037] Table two is the effect that this embodiment is applied to prawn earthen pond culture:

[0038] Table 2. Application effect of compound preparation earth pond

[0039] project value NH before administration 4 + -N (mg / L)

[0040] Remarks: a, the breeding process was 101 days, and the compound preparation was used 5 times in total, with an average interval of 20 days; b, except for the compound preparation, the other...

Embodiment 3

[0042] The application case of shrimp farming in small sheds. This embodiment includes component A and component B, wherein component A is 50% of Paracoccus holophagous, 25% of Pseudomonas, and 25% of Marinobacter spp. Component B is vanillin 5%, acetyltransferase 5%, sodium nitrate 20%, activated carbon powder 5%, calcium hydroxide 15%, polyacrylamide 30%, sodium lauryl sulfate 5%, sodium percarbonate 15% %. The dosage of component B is 2 mg / m 3 Water body; the dosage of component A after expansion is 0.1 mL / m 3 Water body.

[0043] Table two is the effect that this embodiment is applied to prawn small shed cultivation:

[0044] Table 3. Application effect of compound preparation small shed

[0045] project value NH before administration 4 + -N (mg / L)

[0046] Remarks: a, the breeding process was 90 days, and the compound preparation was used 3 times, with an average interval of 30 days; b, except for the compound preparation, the other operations we...

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Abstract

The invention discloses a compound biological agent for improving the survival rate of shrimp culture and a use method of the compound biological agent. The agent mainly comprises a component A and acomponent B, the component A is prepared from the following components in percentage by mass: 30-50% of paracoccus pantotrophus and 50-70% of aerobic denitrification bacteria; and the component B is prepared from the following components in percentage by mass: 10-20% of a quorum-sensing quenching agent, 10-15% of a synergist, 20-30% of a flocculant, 10-15% of a surfactant and 20-30% of an oxygenate producer. According to the compound biological agent, the component B is used first, and at the same time, the component A is used later. Redox potential of the bottom of a culture environment can be improved by the preparation, through cooperative and sequential application of the component A and the component B, based on the principles of quorum-sensing quenching, microbial ecological positionoccupation, microbial matrix competition, etc., harmful floras in the bottom environment are reduced, so generation of hydrogen sulfide, ammonia nitrogen, nitrite and other toxic substances is basically eliminated, and thus the survival rate of the shrimp culture is improved. Compared with conventional biological agents and chemical agents, the compound biological agent has the advantages of quick effect and high sustainability.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and specifically relates to a compound biological agent preparation technology and a use method for improving the survival rate of prawn culture by improving the culture bottom environment. Background technique [0002] Prawns (mainly Litopenaeus vannamei, Litopenaeus vannamei ;Penaeus monodon, Penaeus monodon ; Japanese prawns, Penaeus japonicus ; Chinese prawns, Penaeus japonicus ) Aquatic products are popular among consumers. Shrimp farming has become one of the means for Chinese aquaculture practitioners to get rich, and it also provides effective support for ensuring the safety of the vegetable basket. [0003] However, due to the large amount of high-protein feed fed during the breeding process, it is very easy to cause toxic ammonia nitrogen, hydrogen sulfide, nitrite and other substances in the bottom environment. Due to its own physiological characteristics, the bottom envir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F9/14C02F101/16C02F103/20
CPCC02F1/50C02F1/52C02F1/68C02F3/34C02F2101/16C02F2101/163C02F2101/166C02F2103/20
Inventor 阮贇杰徐向阳孔德栋殷旭旺蔡磊陆慧锋周凡丁雪燕
Owner ZHEJIANG UNIV
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