Radix astragali, flos lonicerae and soybean meal combined fermentation product and livestock and aquatic animal breeding feed
A technology combining fermentation and honeysuckle, applied in animal feed, animal feed, animal husbandry, etc., can solve problems affecting human health, drug residues, antibiotic resistance, etc., to improve immunity, prevent disease, and prevent residual poisoning. Effects with few side effects
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Embodiment 1
[0031] A combined fermentation product of astragalus, honeysuckle and soybean meal, which is prepared by the following steps:
[0032] a) Strain culture: Saccharomyces cerevisiae was cultured with YPDA medium at 30°C and 150rpm for 8 hours to obtain a yeast liquid, and Bacillus subtilis was cultured with LB medium at 30°C and 150rpm for 24 hours. To obtain Bacillus subtilis bacteria liquid, Lactobacillus acidophilus was cultured at 36°C for 48 hours using MARS medium to obtain Lactobacillus acidophilus liquid, and Enterococcus faecalis was cultured at 36°C for 48 hours to obtain feces Enterococcus bacteria liquid; after culture, the concentration of yeast is 1×10 7 CFU / mL, the concentration of Bacillus subtilis is 1×10 8 CFU / mL, the concentration of Lactobacillus acidophilus is 1×10 9 CFU / mL, the concentration of Enterococcus faecalis is 1×10 9 CFU / mL;
[0033] b) Preparation of raw materials: prepare raw materials according to the following formula, honeysuckle 3g, astrag...
Embodiment 2
[0038] A combined fermentation product of astragalus, honeysuckle and soybean meal, which is prepared by the following steps:
[0039] a) Strain culture: Saccharomyces cerevisiae was cultured with YPDA medium at 28°C and 140rpm for 7.5 hours to obtain a yeast liquid, and Bacillus subtilis was cultured with LB medium at 28°C and 140rpm for 22 hours. To obtain Bacillus subtilis bacteria liquid, culture Lactobacillus acidophilus using MARS medium at 34°C for 44 hours to obtain Lactobacillus acidophilus liquid, and use MARS medium to culture Enterococcus faecalis at 34°C for 44 hours to obtain feces Enterococcus bacterial liquid; after strain culture, the concentration of yeast is 5×10 7 CFU / mL, the concentration of Bacillus subtilis is 5×10 8 CFU / mL, the concentration of Lactobacillus acidophilus is 5×10 9 CFU / mL, the concentration of Enterococcus faecalis is 5×10 9 CFU / mL;
[0040] b) Preparation of raw materials: prepare raw materials according to the following formula, hon...
Embodiment 3
[0045] A combined fermentation product of astragalus, honeysuckle and soybean meal, which is prepared by the following steps:
[0046] a) Strain cultivation: Saccharomyces cerevisiae was cultured with YPDA medium at 32°C and 160rpm for 8.5 hours to obtain a yeast liquid, and Bacillus subtilis was cultured with LB medium at 32°C and 160rpm for 26 hours. To obtain Bacillus subtilis bacteria liquid, Lactobacillus acidophilus was cultured at 38°C for 52 hours using MARS medium to obtain Lactobacillus acidophilus liquid, and Enterococcus faecalis was cultured at 38°C for 52 hours to obtain feces Enterococcus bacteria liquid; after culture, the concentration of yeast is 1×10 8 CFU / mL, the concentration of Bacillus subtilis is 1×10 9 CFU / mL, the concentration of Lactobacillus acidophilus is 1×10 10 CFU / mL, the concentration of Enterococcus faecalis is 1×10 10 CFU / mL;
[0047] b) Preparation of raw materials: prepare raw materials according to the following formula, 4 parts by wei...
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