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Preparation method of a controlled-release hydrogel with photothermal therapy and wound repair functions

A technology of wound repair and photothermal therapy, which is applied in the field of preparation of controlled release hydrogel, can solve the problems of inability to remove tumors and tumor recurrence, achieve relief or side effects, simple experimental operation process, and reduce dosage Effect

Active Publication Date: 2022-04-01
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of tumors in our country is still based on surgical resection, but most of the operations cannot completely remove the tumor, which leads to tumor recurrence.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1) Add 1g of alginic acid to 47mL of MES buffer and stir for 2h;

[0038] 2) Dissolve 85 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 51 mg of N-hydroxysuccinimide in 1 mL of MES buffer respectively;

[0039] 3) Put the solution in step 1) in a water bath and heat up to 60°C, and add the 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloric acid solution in step 2) under nitrogen protection and N-hydroxysuccinimide solution. Stir for 1 h, during which the pH of the solution is maintained at 5 with hydrochloric acid or sodium hydroxide;

[0040] 4) Finally, add 550 mg of dopamine to the solution obtained in step 3), and then react for 12 hours under stirring, and maintain the pH of the solution at 5 with hydrochloric acid or sodium hydroxide during stirring;

[0041] 5) The solution obtained in step 4) was placed in an 8000 Da dialysis bag and dialyzed in deionized water in an acidic environment for 2 days, then frozen in an ultra-low temp...

Embodiment 2

[0050] 1) Add 1.25g of alginic acid to 47.5mL of MES buffer, and stir for 2h;

[0051] 2) Dissolve 87.5 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 53 mg of N-hydroxysuccinimide in 1.25 mL of MES buffer respectively;

[0052] 3) Put the solution in step 1) in a water bath and heat up to 60°C, and add the 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloric acid solution in step 2) under nitrogen protection and N-hydroxysuccinimide solution. Stir for 1.25h, and maintain the pH of the solution at 5 with hydrochloric acid or sodium hydroxide during stirring;

[0053] 4) Add 570 mg of dopamine to the solution obtained in step 3), and react under stirring for 14 hours. During the stirring, use hydrochloric acid or sodium hydroxide to maintain the pH of the solution at 5;

[0054] 5) The solution obtained in step 4) was placed in an 8000 Da dialysis bag and dialyzed in deionized water in an acidic environment for 2 days, then frozen in an ultra-low ...

Embodiment 3

[0063] 1) Add 1.5g of alginic acid to 48mL of MES buffer, and stir for 2h;

[0064] 2) Dissolve 90mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 55mg of N-hydroxysuccinimide in 1.5mL of MES buffer respectively;

[0065]3) Put the solution in step 1) in a water bath and heat up to 60°C, and add the 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloric acid solution in step 2) under nitrogen protection and N-hydroxysuccinimide solution. Stir for 1.5h, and maintain the pH of the solution at 6 with hydrochloric acid or sodium hydroxide during stirring;

[0066] 4) Add 600 mg of dopamine to the solution obtained in step 3), and then react for 16 hours under stirring, and maintain the pH of the solution at 6 with hydrochloric acid or sodium hydroxide during stirring;

[0067] 5) The solution obtained in step 4) was placed in an 8000 Da dialysis bag and dialyzed in deionized water in an acidic environment for 3 days, then frozen in an ultra-low temperatu...

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Abstract

The invention relates to the field of medical polymers, and discloses a method for preparing a controlled-release hydrogel with photothermal therapy and wound repair functions. The invention first modifies dopamine on alginic acid so that its molecular chain has an o-diphenol side At the same time, copper sulfide@melano-PEG@dopamine nanoparticles with good photothermal properties and radiotherapy effects were prepared; then copper sulfide@dopamine nanoparticles were added to the dopamine-modified alginic acid solution, and at the same time added Astragaloside IV, which has the effect of promoting wound healing, is then catalyzed by horseradish peroxidase and hydrogen peroxide to form a gel. The hydrogel has good controlled-release performance, and can play the role of reducing dosage and alleviating or avoiding toxic and side effects. The hydrogel prepared by the method has good biocompatibility, uses photothermal properties and radiotherapy to treat cancer cells, and combines the effect of astragaloside IV to promote postoperative wound healing.

Description

technical field [0001] The invention relates to the field of medical polymer materials, in particular to a method for preparing a controlled-release hydrogel with functions of photothermal therapy and wound repair. Background technique [0002] As a major disease that threatens human life and health, cancer has long been characterized by low cure rate, high recurrence rate and high mortality rate. At present, the treatment of tumors in my country is still dominated by surgical resection, but most operations cannot completely remove the tumor, which leads to tumor recurrence. After surgical resection of the tumor, in order to remove the remaining tumor tissue, we need a treatment method that can act in a small local area and with milder conditions to achieve this goal. Undoubtedly, photothermal therapy that can target specific areas and have mild conditions is a more suitable choice. At the same time, slow postoperative wound healing is also one of the problems that needs to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/06A61K47/36A61K47/34A61K41/00A61K31/7048A61K51/12A61K51/06A61P35/00A61P17/02
CPCA61K9/06A61K9/0014A61K47/36A61K47/34A61K41/0052A61K31/7048A61K51/1213A61K51/06A61P35/00A61P17/02A61K2300/00
Inventor 王秉金小康蒋鹏邱方燚万军民
Owner ZHEJIANG SCI-TECH UNIV
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