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Imaging method of thyroid tissue structure

An imaging method and tissue structure technology, applied in the field of biomedicine, can solve problems such as one-sided evaluation indicators, difficulty in obtaining tissue microstructure information, and inability to collect three-dimensional and complete gland information, achieving high specificity and reducing fluorescence loss , deep penetration effect

Active Publication Date: 2019-11-05
SHANDONG PROVINCIAL HOSPITAL
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Problems solved by technology

However, the above three methods have their obvious limitations: gross observation can only evaluate the appearance of the whole gland, and it is difficult to obtain tissue microstructure information; although ultrasound imaging can reflect the internal blood flow and structure of the tissue, but due to the The characteristics of the image and the evaluation index are too one-sided; the pathological examination is significantly better than the above two methods in terms of microscopic evaluation, but because this method is based on tissue slices, it cannot collect three-dimensional and complete gland information

Method used

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  • Imaging method of thyroid tissue structure
  • Imaging method of thyroid tissue structure

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Embodiment 1

[0043] Embodiment 1 provides a kind of imaging method of thyroid tissue structure, comprises the following steps:

[0044] (1) Prepare fluorescently labeled cholesterol solution: 1000g, centrifuge the reagent bottle for 1min, centrifuge 10mg NBD-cholesterol powder to the bottom of the tube; turn off the fan in the ultra-clean bench, keep away from the infrared light, add 10ml of absolute ethanol, quickly close the lid tightly, and shake Mix well to obtain 1 mg / ml NBD-cholesterol absolute ethanol mother solution, which is transparent yellow-green; aseptically dispense into high-pressure sterilized EP tubes, and store in the dark at -30°C; use absolute ethanol to dilute 100 μl of Dilute the 1mg / ml NBD-cholesterol anhydrous ethanol mother solution to 500μl for later use, and make it now.

[0045] (2) Anesthetize and expose the carotid sheath: rats were anesthetized by intraperitoneal injection of 3% pentobarbital sodium (1ml / kg body weight). After full anesthesia, 75% alcohol was...

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Abstract

The invention provides an imaging method of a thyroid tissue structure. The imaging method comprises the steps that (1) fluorescence marking lipid is used to mark thyroid tissue; and (2) a fluorescence microscope is used to perform scanning imaging on the thyroid tissue so as to display a follicle structure of the thyroid tissue. According to the imaging method of the thyroid tissue structure, bythe adoption of the fluorescence marking lipid, a thyroid cell membrane is made to load the lipid and marked with fluorescence, a dynamic and clear thyroid tissue microscopic structure is provided, and the imaging method can be widely applied to assessment of the thyroid tissue structure of a thyroid disease animal model and has great significance for formulation of a research and control strategyabout pathogenesis of a thyroid disease.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an imaging method of thyroid tissue structure, which can be used to evaluate the microscopic morphology of thyroid tissue structure in various animal models of thyroid diseases such as hypothyroidism and hyperthyroidism. Background technique [0002] In recent years, the prevalence of thyroid disease has increased significantly worldwide. From 1988 to 1994, 17,353 people were surveyed for health and nutrition in the United States, and the detection rates of hypothyroidism and hyperthyroidism were 4.6% and 1.3%, respectively. In 2010, the results of the first epidemiological survey of thyroid diseases among residents of ten cities in China showed that the prevalence rates of hypothyroidism and hyperthyroidism also remained high. An editorial in the internationally renowned magazine Lancet in 2012 pointed out that the whole world is facing the threat of thyroid dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/28A61D7/00
CPCG01N21/64G01N1/28A61D7/00
Inventor 邵珊珊赵家军高聆张晓寒
Owner SHANDONG PROVINCIAL HOSPITAL
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