Composite amplification system and kit for parting detection of clopidogrel, statin and aspirin related drug metabolic genes

Abstract

The invention discloses a composite amplification system and kit for parting detection of clopidogrel, statin and aspirin related drug metabolic genes. The composite amplification system comprises twoforward primers and one revere fluorescent primer of each of eight SNP detection sites, and the eight SNP detection sites can be simultaneously amplified. The composite amplification system has the characteristics that through a multiple fluorescent Arms-PCR technology, the eight SNP detection sites are amplified through one tube, and clopidogrel, statin and aspirin personalized drugs can be subjected to auxiliary diagnosis in a combined mode through a detection result; especially, the amplification system can realize direct amplification of blood and blood card samples, and the step of extracting DNA is omitted; the amplification system can integrate UDG-dUTP anti-pollution measures, and product pollution can be prevented effectively; and the detection system is comprehensive in detection site, easy and convenient to operate, high in specificity, high in sensitivity, high in reliability and low in cost, and has the ability of large-batch detection.

Description

technical field [0001] The invention relates to a technique based on multiple fluorescent PCR and capillary electrophoresis. Using this technology to simultaneously detect the polymorphisms of 8 sites in the 4 genes of CYP2C19, ApoE, SLCO1B1 and PTGS1 related to the drug metabolism rate of clopidogrel, statin and aspirin, it provides clinical information for individualized medicine in the treatment of cardiovascular and other diseases. Reference, belongs to the clinical molecular detection technology in the field of biomedicine. Background technique [0002] The incidence of cardiovascular disease is increasing year by year all over the world, and has become the number one killer affecting human health. However, due to individual differences, the therapeutic effects of the drugs commonly used in clinical practice for cardiovascular diseases are obviously different for different patients taking the same dose of drugs, which makes clinicians have certain troubles when using t...

AI Technical Summary

Problems solved by technology

The main disadvantages of this method are: (1) Before the PCR reaction, DNA needs to be extracted from the sample to detect the quality and concentration, the operation is complicated, and there is a risk of confusing the sample

(2) When detecting multiple sites, one sample needs to perform multiple reactions, the detection cost is high, the demand for the amount of template is high, the operation intensity is high, and it is easy to cause operation errors and pollution

Generally speaking, the steps are cumbersome, the test period is long, and the cost is high, which is not conducive to large-scale clinical promotion

2. RFLP analysis method: Based on the change of the restriction endonuclease recognition site caused by gene mutation, such as the loss of the site or the generation of

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