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Small aerobic bacteria culture device and aerobic bacteria culture method

A culture device and aerobic bacteria technology, applied in the field of microbial culture, can solve the problems of easy pollution in the production process, difficulty in concentrating the bacterial solution, and excessive sewage discharge, and achieve the effects of shortening the domestication time, ensuring normal growth, and convenient operation

Pending Publication Date: 2019-11-12
山东农微生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the field of pure culture fermentation of large quantities of aerobic bacteria or facultative aerobic bacteria, the traditional fermenter liquid aeration culture method is mostly used. This method generally has the disadvantages of easy pollution in the production process, low production efficiency, difficulty in concentrating the bacterial liquid, and sewage discharge. Bottlenecks such as many and high production costs

Method used

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  • Small aerobic bacteria culture device and aerobic bacteria culture method
  • Small aerobic bacteria culture device and aerobic bacteria culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A small-sized aerobic bacteria cultivation device, comprising a thermal insulation tank 1, a liquid storage bottle 2, a ventilation pipe 4, an exhaust pipe 5, a liquid injection pipe 6, a syringe 7, a temperature controller 8, a temperature measuring probe 9, and a heating probe 10 , the liquid storage bottle 2 is placed in the heat preservation water tank 1, the bottleneck of the liquid storage bottle 2 is provided with a bottle stopper 3, and the ventilation pipe 4, the exhaust pipe 5, and the liquid injection pipe 6 pass through the bottle in sequence. The plug 3 extends into the liquid storage bottle 1; the vent pipe 4 extends into the bottom of the liquid storage bottle 2; the exhaust pipe 5 extends above the liquid level of the liquid in the liquid storage bottle 2 position; the liquid injection pipe 7 extends into the position below the liquid level of the liquid in the liquid storage bottle 2; a temperature controller 8 is provided on one side of the heat preserv...

Embodiment 2

[0032] A small-sized aerobic bacteria cultivation device, comprising a thermal insulation tank 1, a liquid storage bottle 2, a ventilation pipe 4, an exhaust pipe 5, a liquid injection pipe 6, a syringe 7, a temperature controller 8, a temperature measuring probe 9, and a heating probe 10 , the liquid storage bottle 2 is placed in the heat preservation water tank 1, the bottleneck of the liquid storage bottle 2 is provided with a bottle stopper 3, and the ventilation pipe 4, the exhaust pipe 5, and the liquid injection pipe 6 pass through the bottle in sequence. The plug 3 extends into the liquid storage bottle 1; the vent pipe 4 extends into the bottom of the liquid storage bottle 2; the exhaust pipe 5 extends above the liquid level of the liquid in the liquid storage bottle 2 position; the liquid injection pipe 7 extends into the position below the liquid level of the liquid in the liquid storage bottle 2; a temperature controller 8 is provided on one side of the heat preserv...

Embodiment 3

[0037] A small-sized aerobic bacteria cultivation device, comprising a thermal insulation tank 1, a liquid storage bottle 2, a ventilation pipe 4, an exhaust pipe 5, a liquid injection pipe 6, a syringe 7, a temperature controller 8, a temperature measuring probe 9, and a heating probe 10 , the liquid storage bottle 2 is placed in the heat preservation water tank 1, the bottleneck of the liquid storage bottle 2 is provided with a bottle stopper 3, and the ventilation pipe 4, the exhaust pipe 5, and the liquid injection pipe 6 pass through the bottle in sequence. The plug 3 extends into the liquid storage bottle 1; the vent pipe 4 extends into the bottom of the liquid storage bottle 2; the exhaust pipe 5 extends above the liquid level of the liquid in the liquid storage bottle 2 position; the liquid injection pipe 7 extends into the position below the liquid level of the liquid in the liquid storage bottle 2; a temperature controller 8 is provided on one side of the heat preserv...

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Abstract

The invention relates to a small aerobic bacteria culture device and an aerobic bacteria culture method. The small aerobic bacteria culture device comprises a heat preservation water tank, a liquid storage bottle, a breather pipe, an exhaust pipe, a liquid injection pipe, an injector, a temperature controller, a temperature measuring probe and a heating probe, the liquid storage bottle is placed in the heat preservation water tank, a bottle plug is arranged in the opening of the liquid storage bottle, and the breather pipe, the exhaust pipe and the liquid injection pipe sequentially penetratethrough the bottle plug to extend into the liquid storage bottle; the breather pipe extends into the bottom of the liquid storage bottle; the exhaust pipe extends to the position above the liquid level of liquid in the liquid storage bottle; the liquid injection pipe extends into the position below the liquid level of the liquid in the liquid storage bottle; and the temperature controller is arranged on one side of the heat preservation water tank, the temperature controller is connected with the temperature measuring probe and the heating probe, and the temperature measuring probe and the heating probe penetrate through the side wall of the heat preservation water tank into the heat preservation water tank. The advantages that the cost is low, simpleness, convenience and practicability are achieved, and the culture environment can be controlled are achieved. According to the small aerobic bacteria culture device and the aerobic bacteria culture method, normal growth of biochemical bacteria is guaranteed through a nutrient solution and an activating agent, the acclimation time of aerobic bacteria is shortened, and the biochemical processing efficiency is improved.

Description

technical field [0001] The application belongs to the technical field of microorganism cultivation, and in particular relates to a small aerobic bacteria cultivation device and an aerobic bacteria cultivation method. Background technique [0002] The cultivation of microorganisms requires four essential basic conditions: culture medium (ie, nutrients), temperature, gas environment, and pH. Aerobic bacteria are a type of microorganisms that have a relatively complete respiratory enzyme system, require molecular oxygen as a hydrogen acceptor, and can only grow and reproduce under aerobic conditions. With the continuous development of biotechnology, the demand for pure culture of aerobic bacteria in the field of microbial fermentation is increasing. [0003] At present, the laboratory culture of aerobic bacteria is a common, simple and mature technology, such as liquid and solid culture; test tube, plate, eggplant-shaped bottle culture, etc., but the process of mass production...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/26C12M1/24C12M1/04C12M1/00C12N1/36
CPCC12M23/08C12M23/38C12M33/04C12M29/20C12M41/12C12M29/04C12N1/36
Inventor 冯艳忠何流琴田立余王兆山吴建李凤兰张友明廖秀东李佳佳杨秀梅赫兰保
Owner 山东农微生物科技有限公司
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