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mRNA vaccine and kit and application thereof

A technology for kits and vaccines, used in DNA/RNA vaccination, vaccines, intact cells/viruses/DNA/RNA components, etc.

Pending Publication Date: 2019-11-15
陈朝兴
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are currently peptide vaccines targeting the two targets of KRas and Survivin, but there have been no reports of successful use of these peptide vaccines in clinical trials

Method used

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  • mRNA vaccine and kit and application thereof
  • mRNA vaccine and kit and application thereof
  • mRNA vaccine and kit and application thereof

Examples

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Embodiment Construction

[0040] Hereinafter, the present invention will be further described in conjunction with the embodiments and drawings, which are only for explanation and not intended to limit the protection scope of the present invention.

[0041] The present invention and embodiment mainly relate to the following contents:

[0042] 1) 6, 10 and 11 epitope antigens (Epitopes) were designed for KRas, Survivin and CEA, respectively, and after mixing, they were cloned into 4 vectors (each vector contained 3 targets), and prepared by in vitro transcription The mRNA was capped to improve the stability of the mRNA, and four mRNA vaccines were prepared after purification.

[0043] 2) CT26 mouse-derived rectal cancer cells only contain two genes, KRas and Survivin. In order to evaluate the three targets at the same time, we constructed a CEA-CT26 stable cell line for the establishment of animal model experiments.

[0044] 3) Using a preventive animal model, add CEA-CT26 rectal cancer cells and the va...

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Abstract

The invention relates to an mRNA vaccine and a kit and application thereof. The mRNA vaccine is prepared from an epitope antigen gene sequence of Kras, an epitope antigen gene sequence of Survivin andan epitope antigen sequence of CEA, wherein the Kras, the Survivin and the CEA are separated. The kit includes the mRNA vaccine and a PD-1 antibody. The antibody can be used for preparing medicine for treatment of diseases such as rectal cancer, liver cancer, pancreatic cancer, breast cancer, bladder cancer, fibrosarcoma, renal cancer, leukemia, lung cancer, white lymphoma, lymphoma, melanoma, myeloma, neuroblastoma, plasmacytoma and prostate cancer. The mRNA vaccine is prepared by combining epitope antigens of the three non-drugable targets including the KRas, the Survivin and the CEA for the first time, thereby achieving the purpose of inhibiting tumor growth.

Description

technical field [0001] The present invention relates to mRNA vaccine and its kit and application. Background technique [0002] Many methods have been tried in the field of cancer treatment, such as tumor resection, but it recurs soon after removal; radiotherapy and chemotherapy can kill fast-growing cancer cells, but the tumor will grow back; small molecule targeted drugs and macromolecular Targeted drugs treat cancer, and drug resistance occurs; CAR-T, as a new cancer treatment method, is mainly effective in the treatment of blood cancer patients; the emergence of PD-1 and PD-L1 antibody drugs represents the beginning of immunotherapy, but Only less than 30% of cancer patients can benefit from it. Therefore, therapeutic mRNA vaccines are a new type of immunotherapy that can effectively inhibit tumor growth. Specifically, mRNA is transported to the cytoplasm through nano-lipid particles, and the human body's own protein translation system is used to produce antigens, whic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61P35/00
CPCA61K39/001182A61P35/00A61K2039/53
Inventor 吴晨衍
Owner 陈朝兴
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