mRNA vaccine and kit and application thereof

A technology for kits and vaccines, used in DNA/RNA vaccination, vaccines, intact cells/viruses/DNA/RNA components, etc.

Pending Publication Date: 2019-11-15
陈朝兴
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are currently peptide vaccines targeting the two targets of KRas and Survivin, but there have been no reports of successful use of these peptide vaccines in clinical trials

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • mRNA vaccine and kit and application thereof
  • mRNA vaccine and kit and application thereof
  • mRNA vaccine and kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0040] Hereinafter, the present invention will be further described in conjunction with the embodiments and drawings, which are only for explanation and not intended to limit the protection scope of the present invention.

[0041] The present invention and embodiment mainly relate to the following contents:

[0042] 1) 6, 10 and 11 epitope antigens (Epitopes) were designed for KRas, Survivin and CEA, respectively, and after mixing, they were cloned into 4 vectors (each vector contained 3 targets), and prepared by in vitro transcription The mRNA was capped to improve the stability of the mRNA, and four mRNA vaccines were prepared after purification.

[0043] 2) CT26 mouse-derived rectal cancer cells only contain two genes, KRas and Survivin. In order to evaluate the three targets at the same time, we constructed a CEA-CT26 stable cell line for the establishment of animal model experiments.

[0044] 3) Using a preventive animal model, add CEA-CT26 rectal cancer cells and the va...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an mRNA vaccine and a kit and application thereof. The mRNA vaccine is prepared from an epitope antigen gene sequence of Kras, an epitope antigen gene sequence of Survivin andan epitope antigen sequence of CEA, wherein the Kras, the Survivin and the CEA are separated. The kit includes the mRNA vaccine and a PD-1 antibody. The antibody can be used for preparing medicine for treatment of diseases such as rectal cancer, liver cancer, pancreatic cancer, breast cancer, bladder cancer, fibrosarcoma, renal cancer, leukemia, lung cancer, white lymphoma, lymphoma, melanoma, myeloma, neuroblastoma, plasmacytoma and prostate cancer. The mRNA vaccine is prepared by combining epitope antigens of the three non-drugable targets including the KRas, the Survivin and the CEA for the first time, thereby achieving the purpose of inhibiting tumor growth.

Description

technical field [0001] The present invention relates to mRNA vaccine and its kit and application. Background technique [0002] Many methods have been tried in the field of cancer treatment, such as tumor resection, but it recurs soon after removal; radiotherapy and chemotherapy can kill fast-growing cancer cells, but the tumor will grow back; small molecule targeted drugs and macromolecular Targeted drugs treat cancer, and drug resistance occurs; CAR-T, as a new cancer treatment method, is mainly effective in the treatment of blood cancer patients; the emergence of PD-1 and PD-L1 antibody drugs represents the beginning of immunotherapy, but Only less than 30% of cancer patients can benefit from it. Therefore, therapeutic mRNA vaccines are a new type of immunotherapy that can effectively inhibit tumor growth. Specifically, mRNA is transported to the cytoplasm through nano-lipid particles, and the human body's own protein translation system is used to produce antigens, whic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61P35/00
CPCA61K39/001182A61P35/00A61K2039/53
Inventor 吴晨衍
Owner 陈朝兴
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap