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Cherokee rose root fingerprint establishing method and cherokee rose root quality detection method

A technique for fingerprinting and establishing methods, which is applied in the field of analysis of Chinese herbal medicines, can solve problems such as weak quality control, uneven quality of rosa chinensis, and large differences in medicinal effects of roe cherulae, achieving accurate and reliable detection, short time-consuming, and time-consuming preparation short effect

Active Publication Date: 2019-11-15
ZHUZHOU QIANJIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for establishing the fingerprint of rosa chinensis in view of the problem that the quality of the existing rosa chinensis is uneven and the quality control is relatively weak, resulting in large differences in the medicinal effects of roe chinensis

Method used

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  • Cherokee rose root fingerprint establishing method and cherokee rose root quality detection method
  • Cherokee rose root fingerprint establishing method and cherokee rose root quality detection method
  • Cherokee rose root fingerprint establishing method and cherokee rose root quality detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The establishment method of embodiment 1 golden cherry root fingerprint collection

[0060] A kind of establishment method of golden cherry root fingerprint spectrum, specifically comprises the following steps:

[0061] (1) Preparation of the test solution: add methanol according to the mass volume ratio of 1:50, heat and reflux to extract, after cooling, use methanol to make up for the weight loss, shake well, filter through a 0.22 μm microporous membrane, and take the filtrate to obtain the test product solution;

[0062] (2) Configuration of reference substance

[0063] The reference substances are: prickly glycoside, rosin, phylloside, rosic acid, policinic acid, glucoside B, 24-deoxy-sericoside, 2-oxo-pomulolic acid, pomulolic acid, ursin fruit acid.

[0064] Preparation process: Take appropriate amount of reference substances of pricklyandroside, pyrosin, phylloside, rosinic acid, and pallinica acid respectively, weigh them accurately, and add methanol to make ...

Embodiment 2

[0099] The mensuration of embodiment 2 golden cherry root composition and content

[0100] There are many types of triterpenoids contained in the root of the golden cherry. Through the test of Example 1, it can be seen that the important active ingredients of the triterpenoids are pricklyside, rosin, phylloside, rosic acid and pallinic acid. Therefore, According to the HPLC detection conditions determined in Example 1, the contents of pricklyside, rosin, phylloside, rosacic acid, and pollenic acid in 20 batches of Rosa radix medicinal materials were determined to evaluate the quality of Rosa roe.

[0101] 1. Determination of linear relationship

[0102] 1) Preparation of reference solution

[0103] The reference substances are: prickly glycoside, rosin, phyllotidic acid, rosic acid, and pallinic acid.

[0104] Preparation process: Take appropriate amount of reference substances of pricklyandroside, pyrosin, phylloside, rosinic acid, and pallinica acid respectively, weigh the...

Embodiment 3

[0128] The mensuration of embodiment 3 golden cherry root moisture, ash and extract

[0129] In this example, the method specified in the Pharmacopoeia was used to detect the water content, ash content and extracts of 20 batches of rosacea root samples. The specific detection process is as follows.

[0130] 1. Moisture determination

[0131] Determination with reference to the moisture determination method (the second method of "Chinese Pharmacopoeia" 2015 edition four general rules 0832), the specific process is:

[0132] Take 2g of Rosa japonica root medicinal powder, spread it flat in a flat weighing bottle that has been dried to constant weight, the thickness is not more than 5mm, weigh it accurately, open the bottle cap and dry at 105°C for 5 hours, cover the bottle cap, and place it in a desiccator. Allow to cool for 30 minutes, accurately weigh, then dry at the above temperature for 1 hour, let cool, and weigh until the difference between two consecutive weighings does...

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Abstract

The invention discloses a cherokee rose root fingerprint establishing method and a cherokee rose root quality detection method. According to the cherokee rose root fingerprint establishing method, cherokee rose root fingerprint can be obtained by preparation of a tested object solution and HPLC analysis detection. The establishing method is simple in operation, short in time for tested object solution preparation, high in precision, repeatability and stability, and accurate and reliable in detection, and includes more characteristic peaks. The cherokee rose root quality detection method can beused to detect quality of cherokee rose root medicinal materials, and provide high quality control standard for medicine production and detection, ensures the stability of product quality, and ensures that medicine use is safe and effective. Besides, contents of five important triterpene active components, including rosa roxburghii glycoside, multiflora rose glycoside, euscaphic acid and Chinesecinquefoil acid, which much influences the drug effect of cherokee rose root are detected and controlled to control the quality and effect of the cherokee rose root medicine.

Description

technical field [0001] The invention relates to the field of analysis of traditional Chinese medicinal materials, and more specifically, relates to a method for establishing a fingerprint of rosa chinensis and a method for detecting the quality of roe chinensis. Background technique [0002] Golden cherry root, also known as golden cherry scorpion, tuogudan, etc., is the root of the dicotyledonous Rosaceae plant Rosalaevigata Michx. , antidiarrheal blood and metrorrhagia leucorrhea". Its taste is bitter, sour, astringent, flat. Return spleen, liver, kidney meridian, have clearing away heat-damp and promoting diuresis, detoxifying and detumescence, promoting blood circulation to stop bleeding, the effect of astringent and astringent. Rosaceae root mainly contains triterpenoids, flavonoids, and tannins, which can treat spermatorrhea, enuresis, dysentery, diarrhea, metrorrhagia, uterine prolapse, hemorrhoids, burns, etc. Commonly used as the main drug in traditional Chinese ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/86
CPCG01N30/02G01N30/8631G01N30/8686
Inventor 张鹏李伏君张英帅白璐伍实花廖端芳李春
Owner ZHUZHOU QIANJIN PHARMA
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