Multicolor up-conversion nanoprobe as well as preparation method and application thereof

Abstract

The invention provides a multicolor up-conversion nanoprobe and a preparation method and an application thereof. The multicolor up-conversion nanoprobe sequentially comprises up-conversion nanoparticles and a silicon dioxide layer from inside to outside, the up-conversion nanoparticle comprises a NaYF4 inner core and a NaYF4 outer shell, and a NaYF4 middle light-emitting layer is arranged betweenthe NaYF4 inner core and the NaYF4 shell; the NaYF4 intermediate light-emitting layer is doped with Y<3+>, Yb<3+>, Tm<3+> and Ho <3+>; wherein multiple photosensitizers are doped between the up-conversion nanoparticles and the silicon dioxide layer and / or in a silicon dioxide layer, the thickness of the silicon dioxide layer is less than 15nm, the outer surface of the silicon dioxide layer is modified with mitochondrial targeting ligands, the multiple photosensitizers comprise at least three photosensitizers, and one of the three photosensitizers is indocyanine green. According to the nanoprobe, through the sensitization effect of ICG and the deep tissue penetrating capacity of 980 nm laser, the prepared nanoprobe based on UCNPs can achieve synergistic PDT treatment on deep tumors.

Description

technical field [0001] The disclosure belongs to the technical field of biomedicine, and relates to a multi-color up-conversion nano-probe, a preparation method and an application. Background technique [0002] The statements herein merely provide background information related to the present disclosure and may not necessarily constitute prior art. [0003] As an exogenous stimulus that can activate hyperthermia, light has the advantages of high spatiotemporal selectivity and few side effects, and has been widely used in photothermal, photodynamic and light-triggered chemical / gene therapy. Photodynamic therapy (PDT) uses photosensitizers (PS) to generate reactive oxygen species (ROSs), which can selectively and irreversibly destroy cancer cells and tumor tissues without damaging adjacent healthy cells. Its clinical application is mainly limited to short tissue penetration depth, easy aggregation of PS molecules and insufficient generation of ROSs. [0004] Lanthanide-doped...

AI Technical Summary

Problems solved by technology

However, to the best of the knowledge of the inventors of this disclosure, ICG is difficult to use for PDT due to its disadvantages such as instability in aqueous solution, rapid clearance, easy self-bleaching, and lack of targets.

According to the research of the inventors of the present disclosure, it is found that the quantum yield and luminous efficiency of UCNPs with four colors are low, which limits the application of PDT on them.

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