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Method for preparing fusidic acid through fermentation by using plant endophytic fungus

A technology of plant endophytic fungi and fusidic acid, which is applied in the field of chemistry and can solve the problems of low yield and high price in the production process

Pending Publication Date: 2019-11-22
QINGDAO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the output of domestic fusidic acid production process is low, and most of the clinical fusidic acid drugs in my country are imported from foreign countries, and the price is expensive

Method used

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  • Method for preparing fusidic acid through fermentation by using plant endophytic fungus
  • Method for preparing fusidic acid through fermentation by using plant endophytic fungus
  • Method for preparing fusidic acid through fermentation by using plant endophytic fungus

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Embodiment

[0016] Example: endophytic fungal strain: Acremonium pilosumF47; strain source: isolated from the flower axis of the broad-leaved ten merits of the plant; culture medium: potato dextrose agar medium; fermentation medium: rice medium—80g rice, 120ml double steamed Water, 0.3% peptone, 500ml Erlenmeyer flask, static culture at 28°C for 40-50 days; extraction: extraction with organic solvent ethyl acetate to obtain the crude extract; separation: column chromatography operation, the crude extract was normalized on silica gel Phase column, stationary phase is 200-300 mesh silica gel, petroleum ether and ethyl acetate are mobile phases, gradient elution (100:0-0:100, v / v), collecting petroleum ether: ethyl acetate = 2:1 ~1:2 fraction, get the crude product of fusidic acid; Purification: Purify the obtained crude product of fusidic acid by preparative high performance liquid chromatography, using methanol and water as the mobile phase, to get the pure product of fusidic acid.

[0017...

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Abstract

The invention discloses a method for preparing fusidic acid through fermentation by using a plant endophytic fungus. The method includes the steps of firstly, carrying out strain culture on the mahonia fortunei endophytic fungus in a strain culture medium; then, carrying out fermentation culture in a fermentation culture medium; extracting the fermentation product with an organic solvent, after extraction solution mixing, carrying out reduced pressure distillation to obtain a fermentation liquor extract, and carrying out column chromatography separation to obtain crude fusidic acid; carrying out chromatography purification on the crude fusidic acid to obtain pure fusidic acid. The preparation method has the advantages of being simple and capable of producing the fusidic acid on a large scale by using the strain.

Description

technical field [0001] The invention belongs to the technical field of chemistry and relates to a method for preparing fusidic acid by fermenting plant endophytic fungi. Background technique [0002] Fusidic acid is an important clinical antibacterial drug. Fusidic acid sodium salt (injection or ointment) is generally used clinically. It has strong penetrating power to tissues and body fluids, and has a long plasma half-life when administered intravenously. Patients tolerated it well. In terms of drug resistance: there is no cross-resistance between fusidic acid and other clinically used antibacterial drugs, and it has good safety. Fusidic acid can often be used in combination with rifampicin, carboxycin, aminoglycosides, quinolones, tetracyclines and other drugs, and its synergistic effect is remarkable. In terms of side effects: patients generally tolerate it well, and the main side effects include gastrointestinal discomfort, nausea, indigestion, and vomiting. There ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/00C12R1/645
CPCC12P33/00
Inventor 娄红祥李刚田川高涵
Owner QINGDAO UNIV
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