Improved mouse liver lipid droplet oil-red O staining method

A dyeing method and oil red technology, which are applied in the preparation of test samples, material analysis and sampling by optical means, etc., can solve the problems of wrong judgment, accurate judgment of the influence of dyeing results, etc., and achieve the effect of improving accuracy.

Inactive Publication Date: 2019-11-22
DALIAN MEDICAL UNIVERSITY
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Problems solved by technology

[0003] However, at present, there is no uniform technical standard for oil red O staining of lipid droplets in mouse liver tissue, and there are differences in various oil red O staining methods and procedures. At the same time, there are still some defects ...

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  • Improved mouse liver lipid droplet oil-red O staining method

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Embodiment 1

[0031] An improved oil red O staining method for mouse liver lipid droplets, said method comprising the steps of:

[0032] 1. Preparation of frozen sections

[0033] 1) Tissue fixation step: fresh mouse liver was fixed with 10% formalin for 5 days;

[0034] 2) Tissue dehydration step: fully soak the fixed mouse liver in 15% sucrose solution for 24 hours, and then fully soak in 30% sucrose solution for 24 hours;

[0035] 3) Tissue slicing step: using a cryostat to slice the dehydrated mouse liver with a thickness of 8 μm-10 μm, and store the tissue slices in a -80° C. refrigerator;

[0036] 2. Oil red O staining of tissue sections

[0037] 1) Take out the frozen tissue sections from the -80°C refrigerator, place them on a staining rack, and dry them with cold air with a hair dryer;

[0038] 2) In the staining jar, fix with 10% formalin-1% calcium chloride fixative solution for 15 minutes;

[0039] Wherein, the preparation method of described 10% formalin-1% calcium chloride...

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Abstract

The invention relates to an improved mouse liver lipid droplet oil-red O staining method, and belongs to the technical field of lipid droplet detection. The method comprises preparing frozen slices bya tissue fixing step, a tissue dehydration step and a tissue section step. In the tissue fixing step, the fresh mouse liver is fixed for 3-10 days by adopting 10% formalin; and in the tissue dehydration step, the fixed mouse liver is soaked in a 15% sucrose solution for 24 hours, and then soaked in a 30% sucrose solution for 24 hours. According to the method, 10% formalin is firstly adopted to fix the mouse liver, and then 15% sucrose solution and 30% sucrose solution are adopted to perform gradient dehydration on the mouse liver, so that a staining result judgment can be determined more accurately.

Description

technical field [0001] The invention relates to an improved oil red O staining method of mouse liver lipid droplet, belonging to the technical field of lipid droplet detection. Background technique [0002] Oil red O fat staining method refers to the method of staining with oil red O in order to show the fat in the tissue in daily pathological diagnosis and scientific research work. Oil red O is a fat-soluble dye, which is highly soluble in fat and can be specific It can color neutral fats such as triglycerides in tissues to show the content and size of lipid droplets in tissues and cells. [0003] However, at present, there is no uniform technical standard for oil red O staining of lipid droplets in mouse liver tissue, and there are differences in various oil red O staining methods and procedures. At the same time, there are still some defects in the traditional oil red O fat staining method. , For example: the existing Oil Red O staining solution tends to leave red crysta...

Claims

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Application Information

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IPC IPC(8): G01N21/84G01N1/28G01N1/30G01N1/42
CPCG01N1/286G01N1/30G01N1/42G01N21/84G01N2001/305
Inventor 孙杰吴英杰米艾冉丽媛李有为
Owner DALIAN MEDICAL UNIVERSITY
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