Improved mouse liver lipid droplet oil-red O staining method

Abstract

The invention relates to an improved mouse liver lipid droplet oil-red O staining method, and belongs to the technical field of lipid droplet detection. The method comprises preparing frozen slices bya tissue fixing step, a tissue dehydration step and a tissue section step. In the tissue fixing step, the fresh mouse liver is fixed for 3-10 days by adopting 10% formalin; and in the tissue dehydration step, the fixed mouse liver is soaked in a 15% sucrose solution for 24 hours, and then soaked in a 30% sucrose solution for 24 hours. According to the method, 10% formalin is firstly adopted to fix the mouse liver, and then 15% sucrose solution and 30% sucrose solution are adopted to perform gradient dehydration on the mouse liver, so that a staining result judgment can be determined more accurately.

Description

technical field

[0001] The invention relates to an improved oil red O staining method of mouse liver lipid droplet, belonging to the technical field of lipid droplet detection. Background technique

[0002] Oil red O fat staining method refers to the method of staining with oil red O in order to show the fat in the tissue in daily pathological diagnosis and scientific research work. Oil red O is a fat-soluble dye, which is highly soluble in fat and can be specific It can color neutral fats such as triglycerides in tissues to show the content and size of lipid droplets in tissues and cells.

[0003] However, at present, there is no uniform technical standard for oil red O staining of lipid droplets in mouse liver tissue, and there are differences in various oil red O staining methods and procedures. At the same time, there are still some defects in the traditional oil red O fat staining method. , For example: the existing Oil Red O staining solution tends to leave red crysta...

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