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Lactobacillus composition and application thereof

A composition, the technology of Lactobacillus, applied in the field of medicine, can solve the problems of difficult effects, difficulty in colonizing vaginal flora with a single strain, and inability to maintain the amount of viable bacteria, and achieve active and stable biological characteristics and strong colonization ability of strains Effect

Pending Publication Date: 2019-12-06
赵湘江 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the existing products on the market are not the dominant vaginal flora of women in my country, their colonization ability is poor, and they cannot maintain a stable amount of viable bacteria, which cannot fully meet the clinical needs of gynecology.
There are many kinds of Lactobacillus in the vagina of healthy women, and it is difficult for a single strain to play a good role in regulating the vaginal flora, even if a single strain has good acid-producing ability and H-producing capacity. 2 o 2 ability, and the ability to adhere to vaginal epithelial cells, but a single strain also has difficulty in recolonizing into the vaginal flora, and it is difficult to play a long-term role

Method used

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  • Lactobacillus composition and application thereof
  • Lactobacillus composition and application thereof
  • Lactobacillus composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, the preparation of Lactobacillus rhamnosus RD-060 freeze-dried bacterial powder

[0037] Lactobacillus rhamnosus RD-060 was grown in broth liquid medium (MRS) at pH 6.5, fermented in a 100-liter scale fermenter, and cultured anaerobically. The bacteria were collected in the early plateau stage, and the number of viable bacteria reached about 1.0×10 9 More than CFU / ml. Collect the thalli by centrifugation, wash with phosphate buffer, and mix with lyoprotectant (including skimmed milk powder and sucrose, etc.). Then, the mixture was freeze-dried in a freeze dryer. Samples were frozen at -40°C for approximately 2 hours, then vacuum dried at -20°C for 20-30 hours, and then dried at 30°C for 3-5 hours. The dry powder is packaged in aluminum foil bags with desiccant and stored refrigerated. The lyophilized powder is counted for live bacteria, and the number of live bacteria should be 1.0×10 10 CFU / g or more.

Embodiment 2

[0038] Embodiment 2, the preparation of Lactobacillus gasseri RD-0046 freeze-dried bacterial powder

[0039] Lactobacillus gasseri RD-0046 was grown in broth (MRS) at pH 6.5, fermented in a 100 liter scale fermenter, and anaerobically cultured. The bacteria were collected in the early plateau stage, and the number of viable bacteria reached about 1.0×10 9 More than CFU / ml. Collect the thalli by centrifugation, wash with phosphate buffer, and mix with lyoprotectant (including skimmed milk powder and sucrose, etc.). Then, the mixture was freeze-dried in a freeze dryer. Samples were frozen at -40°C for approximately 2 hours, then vacuum dried at -20°C for 20-30 hours, and then dried at 30°C for 3-5 hours. The dry powder is packaged in aluminum foil bags with desiccant and stored refrigerated. The lyophilized powder is counted for live bacteria, and the number of live bacteria should be 1.0×10 10 CFU / g or more.

Embodiment 3

[0040] Embodiment 3, the preparation of composition

[0041]Take 100g of Lactobacillus rhamnosus RD-060 freeze-dried bacterial powder, 100g of Lactobacillus gasseri RD-0046 bacterial powder and mix them with 2.3kg of microcrystalline cellulose and 10g of magnesium stearate, and fill them into No. 2 hard capsules. Each capsule is filled with 220mg~250mg, made into a lactobacillus composition capsule, and stored in cold storage. Count and confirm that the number of live bacteria of a single bacterium should be 1.0×10 8 More than CFU / g.

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PUM

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Abstract

The invention discloses a lactobacillus composition containing a lactobacillus rhamnosus strain and a lactobacillus gasseri strain, and the lactobacillus composition can resist bacterial vaginosis andvarious vaginal infections. The composition disclosed by the invention has obvious effects on bacterial vaginal diseases, is safe and non-toxic, has good stability, and can be stored for a long time.Compared with other currently commercially available preparations, the strains in the composition can be effectively colonized, can work synergistically after implantation in the vagina, can form a vaginal microecology, and have more obvious effects than a single strain.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a lactobacillus composition and its application, especially for the preparation of medicines for treating gynecological diseases. Background technique [0002] There are a variety of microorganisms in the vagina of healthy women, and they form a vaginal micro-ecological system that restricts, coordinates and dynamically balances with the host and the environment. The vaginal flora of healthy women is mainly composed of Lactobacillus, including Lactobacillus rhamnosus, Lactobacillus jansneri, Lactobacillus gasseri, Lactobacillus crispatus, and Lactobacillus vaginalis. Under normal circumstances, Lactobacillus can protect the vagina. When the Lactobacillus in the vaginal microecology is disturbed, it can lead to the invasion of pathogenic bacteria and cause vaginitis. [0003] The occurrence of bacterial vaginosis (BV) is due to the imbalance of vaginal flora, the red...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61P15/02A61P31/04A61P31/10A61P15/00A61P31/12A61P13/00
CPCA61K35/747A61P15/02A61P31/04A61P31/10A61P15/00A61P31/12A61P13/00A61K2300/00Y02A50/30
Inventor 赵湘江潘立
Owner 赵湘江
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