Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Catalpa bungei embryonic callus culture method based on stem segment with leaves

A technology of embryogenic callus and culture method, which is applied in the field of bioengineering, can solve rare problems and achieve the effects of short induction time, simple operation, and high induction efficiency

Inactive Publication Date: 2019-12-13
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI +1
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many researches on the tissue culture technology of Chinese catalpa, but there are few studies on the induction of embryogenic callus of Chinese catalpa.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Catalpa bungei embryonic callus culture method based on stem segment with leaves
  • Catalpa bungei embryonic callus culture method based on stem segment with leaves
  • Catalpa bungei embryonic callus culture method based on stem segment with leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0025] (1) Cultivation of catalpa seedlings in tissue culture

[0026] Wrap the seeds of the mature catalpa strain Q301 with gauze, wash them with tap water for 30 minutes, sterilize the surface with 75% alcohol for 60 seconds, wash them twice with sterile water, then soak them in 0.1% mercuric chloride solution for 18 minutes, then wash them with sterile water. After washing for 5 times, the seed coats at both ends of the seeds were cut and inoculated on the seedling medium, and cultured at 25-28° C., light intensity 1500 lx, and 12 h / d light.

[0027] (2) Induction of callus based on leafy stem segment

[0028] After the seedlings were cultured for 30 days, the stems were cut into 2-3 cm leafy stem segments, inserted into the callus induction medium, and cultured at 25-28 ° C, light intensity 1500 lx, 12 h / d under light, 20 days. Pale yellow callus can grow. Such as figure 1 , figure 2 shown.

[0029] (3) Induction of embryogenic callus

[0030] The induced callus wa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a catalpa bungei embryonic callus culture method based on a stem segment with leaves. According to the method, a stem of a catalpa bungei seedling growing for 30 days under a tissue culture condition is cut into the 2-3-centimeter stem segment with the leaves, the stem segment is inserted into a callus-inducing culture medium to obtain callus, the induced callus is transferred into an embryonic callus-inducing culture medium to induce embryonic callus, and finally the induced embryonic callus is transferred into a proliferation culture medium for proliferation. The catalpa bungei embryonic callus culture method based on the stem segment with the leaves provides important technical support for cultivating high-quality and resistant new species and verifying the gene functions of catalpa bungei.

Description

technical field [0001] The invention relates to a method of bioengineering technology, in particular to a plant tissue culture technology, in particular to the induction of embryogenic callus. Background technique [0002] catalpa( Catalpa bungei ) is a catalpa (Bignoniaceae) genus ( Catalpa Scop.) precious tree species. Catalpa has a tall tree with a dense crown, and its branches, leaves, and flowers all have high ornamental value; the wood is tough, rot-resistant, wear-resistant, moisture-proof, and not easy to be eaten by insects, so it has extremely high economic value. However, in recent years, catalpa catalpa has been severely damaged by diseases and insect pests, which limits its large-scale promotion. Therefore, improving the resistance to diseases and insects of Chinese catalpa and cultivating new varieties of high-quality Chinese catalpa against diseases and insects are the main problems that need to be solved urgently at present. [0003] The traditional gene...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 吕芬妮王鹏高露璐杨如同李亚汪庆张恩亮杨桂娟
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com