Method for inducing ammopiptanthus callus formation by utilizing young and tender ammopiptanthus lateral roots

Abstract

The invention discloses a method for inducing ammopiptanthus callus formation by utilizing young and tender ammopiptanthus lateral roots. The method comprises the following steps: a, performing sterile seedling raising by utilizing ammopiptanthus seeds, and continuously inducing formation of lateral roots with root hair through a 1 / 2MS culture medium; and b, shearing young and tender hair lateral roots as explants, inducing callus formation on a CDB1 culture medium in the dark, and performing subculture and expanding propagation on the induced callus on a CDB6-2 culture medium under the light or in the dark. With the adoption of the method disclosed by the invention, the ammopiptanthus callus can be efficiently and stably obtained at low cost.

Description

technical field [0001] The invention relates to a method for inducing callus formation of holly holly by using young lateral roots of holly holly. Background technique [0002] Sand holly belongs to the genus Sand holly in the leguminous family. It is an evergreen shrub in a super-xerophytic desert, which is suitable for various extreme environments in the desert (drought, high temperature, low temperature, and wind erosion, etc.), and it is also an ancient relic species of the Tertiary period. , is an endangered species, so Ilex is a plant with good stress resistance and beneficial gene resources, and has the value of genetic evolution research on genes and plant biological traits. The predecessors only carried out a lot of research work on its natural protection, community distribution, biological characteristics and genetic background analysis. Until recently, some research reports were about the gene cloning and transcriptome sequencing analysis of Ilex chinensis. It is...

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Problems solved by technology

[0003] However, due to its long growth cycle, low seed setting rate and germination rate, and its vulnerability to insect damage, its reproductive ability is weak; its mature tissues are relatively aging, and the germinated seedlings are also prone to aging and lignification in the later stages of growth, which is not conducive to DNA. and RNA extraction, etc., and researchers cannot plant and breed Ilex japonica indoors, and have to pick a large number of new seeds every year for scientific research, which largely limits the application of molecular biotechnology in gene cloning and functional research of Ilex japonica

At present, there is no patent report about the tissue culture and callus induction of Ilex chinensis, only some Chinese periodicals have a small amount of reports about the induction of Ilex spp. callus, but the induction efficiency is low and unstable (3% to 80%). poor repeatability

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