A Lactobacillus plantarum yw11 with high adsorption and high tolerance to heavy metal lead
A technology of Lactobacillus plantarum and YW11, which is applied in the direction of bacteria, microorganisms, and methods based on microorganisms, can solve the problems of limiting practical application value, high input cost, and increasing secondary pollution, so as to reduce heavy metal pollution and ensure environmental safety. The effect of strong lead adsorption advantage
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Embodiment 1
[0024] Embodiment 1, Lactobacillus plantarum YW11 is to the adsorption capacity of lead
[0025] Strains to be tested: 4 strains of Lactobacillus plantarum (YW11, SKT109, K25 and YNF-5) and 4 strains of Lactococcus lactis (YNK-1-1, XZ16302, XZ35305 and QH40-5).
[0026] 1. Dilute the lead nitrate solution containing lead ion concentration 1g / L in ultrapure water to make the lead ion concentration 20mg / L to obtain lead solution. At the same time, 0, 2.5mg / L, 5mg / L, 10mg / L, 20mg / L, and 40mg / L standard products were prepared with 1g / L standard lead ion aqueous solution to detect the standard curve.
[0027] 2. Inoculate the strain to be tested in liquid MRS medium, and culture it at 37° C. and 200 rpm for 18 hours to activate it.
[0028] 3. Centrifuge the bacteria solution activated in step 2 at 8000 g for 20 minutes to collect the bacterial precipitate, wash the bacterial precipitate with ultrapure water twice, and obtain the bacterial precipitate to be tested.
[0029] 4. We...
Embodiment 2
[0032] Embodiment 2, the tolerance of plantarum lactobacillus YW11 to lead
[0033] Strains to be tested: 4 strains of Lactobacillus plantarum (YW11, SKT109, K25 and YNF-5) and 4 strains of Lactococcus lactis (YNK-1-1, XZ16302, XZ35305 and QH40-5).
[0034] 1. After filtering and sterilizing the lead nitrate solution containing lead ion concentration of 5g / L with a filter membrane with a pore size of 0.22μm, add it to the MRS solid medium to make the concentration of lead ions in the MRS solid medium reach 100mg / L. 500mg / L or 1000mg / L.
[0035] 2. The tolerance of lactic acid bacteria to lead is determined by the minimum inhibitory concentration (minimum inhibitory concentrations, MIC) experiment: the lowest concentration of lead at which lactic acid bacteria do not grow completely in a medium containing lead ions is considered to be MIC. Inoculate the activated strain to be tested in the medium prepared in step 1, culture at 37°C for 48 hours, and count the plate to determin...
Embodiment 3
[0041] Embodiment 3, the influence of lead on the growth of Lactobacillus plantarum YW11
[0042] 1. Filter and sterilize 1g / L lead nitrate solution with a filter membrane with a pore size of 0.22μm, and then add it to the MRS liquid medium to make the concentration of lead ions in the MRS liquid medium reach 50 mg / L.
[0043] 2. Inoculate 50 μl of the activated bacterial solution to be tested in 50 ml of the culture medium prepared in step 1, incubate at 37°C and 200 rpm for 48 hours, and use a spectrophotometer to determine the OD at different time points 600nm The absorbance value reflects the growth status of the strain. Simultaneously, a control of inoculating the strain to be tested in the MRS liquid medium without lead ions for cultivation was set.
[0044] The result is as figure 2 shown. figure 2 Among them, the abscissa is the culture time (h); the ordinate is the OD 600nm absorbance value.
[0045] The results showed that lead ions had varying degrees of infl...
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