Preparation of nanocomposite materials and its application in combined toxicity detection of chlorpyrifos pesticide residues
A nano-composite material and chlorpyrifos pesticide technology, applied in the preparation of Co3O4-His-GQD@RGO nano-composite material, the preparation of nano-composite material and its joint toxicity detection of chlorpyrifos pesticide residues, which can solve the problem of poor conductivity and limited application, etc. problems, to achieve the effect of improving electrical conductivity, preventing agglomeration, and enhancing electrochemical performance
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Embodiment 1
[0033] Example 1Co 3 o 4 -Preparation method of His-GQD@RGO nanocomposite
[0034] The reaction mechanism is as figure 1 shown.
[0035] (1) Preparation of His-GQD: Take raw materials according to citric acid: histidine molar ratio 1:0.1, mix the two evenly, add until water dissolves, 500Hz ultrasonic, 1000r / min stirring, until uniform dispersion; Solution, react in an oven at 160°C for 2 hours to prepare histidine functionalized graphene quantum dots His-GQD;
[0036] The prepared histidine functionalized graphene quantum dots His-GQD were characterized, as shown in Figure 2. in Figure 2-A It is the Raman spectrum of His-GQD, in the figure 1340cm -1 and 1590cm -1 Each has a characteristic peak, 1340cm -1 The D band at is the disordered carbon sp 3 The characteristic peak of the hybrid structure, 1590cm -1 The G band at the carbon sp 2 Characteristic peaks of the hybrid structure. Figure 2-B It is the infrared spectrum of His-GQD, it can be observed from the spec...
Embodiment 2
[0042] Example 2Co 3 o 4 -Preparation of His-GQD@RGO Nanocomposite Electrochemical Biosensor
[0043] (1) Co that will be collected 3 o 4 -His-GQD@RGO nanocomposite was washed 4 times with ultrapure water, and then dispersed in 2 mL of Tris-HCl buffer with pH=7.
[0044] (2) Co obtained in step (1) 3 o 4 -His-GQD@RGO dispersion and 3% chitosan solution with a volume ratio of 30:1 were mixed to obtain a mixed solution C, and 8 μL of the mixed solution C was added dropwise on the surface of the glassy carbon electrode sensor, and then the N 2 Moderately dry conditions. Then immerse the modified glassy carbon electrode in 5mM folic acid activated with 0.1M N-N-hydroxysuccinimide-0.4M ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride mixture, keep 5 hours and rinsed with deionized water to obtain folic acid / Co 3 o 4 -His-GQD@RGO glassy carbon electrode.
Embodiment 3
[0045] Application of Example 3 in the Joint Toxicity Detection of Chlorpyrifos Pesticide Residues
[0046] (1) Cell culture: human gastric cancer cells (BEL-7402) grown to the logarithmic phase after treatment (BEL-7402 cells were cultured in RPMI-1640 medium at 37°C, containing 5% CO 2 10% fetal bovine serum was supplemented in the humid atmosphere, and the cells reached the logarithmic growth phase in the growth retardation stage after 3 days), and the human gastric cancer cells grown to the logarithmic phase after treatment were taken, and 0.5% volume concentration chlorpyrifos solution was added thereto .
[0047] The chemical formula of chlorpyrifos is as follows:
[0048]
[0049] Chlorpyrifos is dispersed in ethanol at a concentration of 0.5%, and the cell base containing 0.5% volume concentration of ethanol is used as the control group of the experiment;
[0050] (2) Dilute 10 μL to a concentration of 3×10 5 cells / mL of human hepatoma cell suspension was added d...
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