Application of taiwanin E in preparation of herbicide
A technology for herbicides and weeds, applied in the field of pesticide research and development, can solve the problems of unreported research on the herbicidal activity of aryl naphthalene lignans
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Embodiment 1
[0029] The separation of embodiment 1 compound Taiwanin E
[0030] After drying and pulverizing the stem bark of the plant, it is extracted with methanol and concentrated to obtain the methanol extract. After the extract was dissolved in methanol and suspended in water, it was sequentially extracted with petroleum ether and ethyl acetate to obtain the ethyl acetate layer extract.
[0031] The ethyl acetate extract was subjected to silica gel column chromatography with chloroform / methanol system, and then combined by TLC. Combined with activity tracking, the active part is subjected to silica gel column chromatography with petroleum ether / acetone and petroleum ether / ethyl acetate in sequence, and then separated by Sephadex LH-20 Sephadex LH-20. After purification, Taiwanin E can be obtained.
Embodiment 2
[0032] The herbicidal activity of embodiment 2 Taiwannin E
[0033] The herbicidal activity of Taiwanin E on different types of weeds was determined by the small cup method, and the results are shown in Table 1.
[0034] Table 1 Herbicidal activity of Taiwanin E (50mg / L, 7d)
[0035] weed species Root length inhibition rate (%) Fresh weight inhibition rate (%) Red thistle (Ageratum conyzoides L.) 100.00±0.00 70.58±0.77 Water lotus grass (Drymaria diandra Blume) 100.00±0.00 71.59±0.37 Nightshade (Solanum nigrum L.) 100.00±0.00 63.37±1.44 Purslane (Portulaca oleracea L.) 94.36±0.47 66.56±2.77 Duckweed (Lemna minor L.) 86.39±0.69 83.71±1.63 Amaranth (Amaranthus retroflexus L.) 74.56±0.40 50.24±1.95 Mikania micrantha Kunth 70.66±2.90 58.72±2.78 Goosegrass (Eleusine indica (L.) Gaertn) 70.49±1.11 42.02±2.05 Ghost needle grass (Bidens pilosa L.) 55.63±1.84 39.51±2.33 Bald barnyard barnyard (Echinochloa c...
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