Antagonistic actinomycetes and bio-organic fertilizer for preventing and controlling tomato bacterial wilt and methods and uses thereof

A bio-organic fertilizer and actinomycetes technology, which is applied in the field of antagonistic actinomycetes and bio-organic fertilizers, can solve problems such as difficulty in survival, and achieve the effects of high price, environmental protection, and quality improvement

Active Publication Date: 2021-06-18
BIOGAS SCI RES INST MIN OF AGRI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The invention provides an antagonistic actinomycete for preventing and controlling tomato bacterial wilt, a bio-organic fertilizer and its method and use. The bio-organic fertilizer solves the problem that the existing bio-control bacteria are difficult to survive after being inoculated into the roots of plants, and can colonize In the rhizosphere of plants, and produce antagonistic substances, inhibit the growth of pathogenic microorganisms in the rhizosphere of plants

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  • Antagonistic actinomycetes and bio-organic fertilizer for preventing and controlling tomato bacterial wilt and methods and uses thereof
  • Antagonistic actinomycetes and bio-organic fertilizer for preventing and controlling tomato bacterial wilt and methods and uses thereof

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Embodiment 1

[0023] 1. Isolation and identification of strains

[0024] Collect tomato planting fields in Chengdu Shuangliu Biogas Science Research Institute Base of the Ministry of Agriculture, select healthy tomato plant rhizosphere soil around the diseased tomato plant to isolate actinomycetes, and use PDA medium (potato dextrose agar medium) to screen the tomato by confrontation method. Antagonistic actinomycetes that have a strong inhibitory effect such as the pathogenic bacteria R. solanacearum of soil-borne root diseases. After re-screening in the pot test, the highly effective antagonistic actinomycete G33 strain was finally obtained, and the biological identification of the strain was carried out. The strain was stored in a 4°C refrigerator and preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee. The preservation number is CGMCC4.7604, the preservation time is July 15, 2019.

[0025] Antagonistic actinomycete G33 belon...

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Abstract

The invention discloses an antagonistic actinomycete for preventing and controlling tomato bacterial wilt, a bio-organic fertilizer, a method and a use thereof. The antagonistic actinomycete G33 is preserved in the General Microorganism Center of the China Microbial Strain Preservation Management Committee, and its preservation number is: CGMCC No.4.7604, the preservation date is July 15, 2019. It belongs to Streptomyces flavinus. It is fermented with biogas residue, cow dung, and rice straw to make bio-organic fertilizer. The bio-organic fertilizer improves soil microbial flora , the antagonistic actinomycete G33 can well colonize the tomato rhizosphere and produce antagonistic substances to inhibit the growth of pathogenic microorganisms in the tomato rhizosphere, thereby significantly reducing the occurrence of tomato bacterial wilt in continuous cropping, with a control rate of over 70%. .

Description

technical field [0001] The invention relates to a biocontrol bacterial strain, in particular to antagonistic actinomycetes for preventing and controlling tomato bacterial wilt, biological organic fertilizer, method and application. Background technique [0002] The mechanism of microbial control of crop diseases is mainly composed of competition, antagonism, quorum sensing inhibition, induction of resistance to plants, and growth promotion. Many studies have shown that the survival and successful colonization of biocontrol bacteria in the plant rhizosphere is the key The key to controlling crop diseases. The traditional biological control technology is to spray the bio-control bacteria into liquid or powder bacterial agents, which can effectively prevent and control the reproduction and invasion of pathogenic bacteria. Harsh conditions make it difficult to colonize and even survive, resulting in unstable control effects and poor sustainability. Therefore, there are certain...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N1/20C05F11/08C05F17/20C05G3/60A01P3/00C12R1/465
CPCC05F3/00C05G3/00C12N1/20C12N1/205C12R2001/465C05F9/04C05F11/00C05F11/08Y02W30/40
Inventor沈婷雷云辉张思洋
OwnerBIOGAS SCI RES INST MIN OF AGRI