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Phenotypic characterization of cells

A cellular, non-cellular technique for such phenotypic characterization of cells to address difficult cell capture or loading issues

Pending Publication Date: 2019-12-20
ASTREGO DIAGNOSTICS AB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] One limitation to conducting efficient microfluidic-based AST is the difficulty of capturing or loading cells into microfluidic devices

Method used

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Embodiment Construction

[0031] Embodiments of the present invention relate generally to phenotypic characterization of cells, and more particularly to such phenotypic characterization of cells in microfluidic devices.

[0032] Microfluidic devices, also known in the art as microfluidic chips, can be used for phenotypic analysis of cells, ie phenotypic characterization, in a time-sensitive manner. However, in real-world applications, the biological sample input into the microfluidic device may contain various biological materials (including target cells to be phenotyped, other cells, and cell debris) as well as non-biological materials (such as dirt, pollutants, etc.) complex heterogeneous samples. Thus, biological samples are often heterogeneous, and cells of interest may actually constitute a minority, or even a very small minority, of the material present in the biological sample.

[0033] Heterogeneity in biological samples can arise for various reasons. For example, the biological sample itself...

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Abstract

Phenotyping of cells involves loading a biological sample into a microfluidic device (1, 100) comprising cell compartments (20, 120) to capture biological material, including target cells, in the sample in the cell compartments (20, 120). A subset (20A)of the cell compartments (20, 120) is identified as comprising target cells exhibiting target phenotype characteristic(s) as determined based on monitoring biological material in the cell compartments (20, 120)prior to addition of a test agent. The biological material is exposed to a test agent and a phenotypic response of the target cells to the test agent is determined based on monitoring target cells in the identified subset (20A)of the cell compartments (20, 120). The phenotyping of the target cells is thereby not overshadowed by the response of other cells and non-cell material present in the biological sample.

Description

technical field [0001] Embodiments of the present invention relate generally to phenotypic characterization of cells, and more particularly to such phenotypic characterization of cells in microfluidic devices. Background technique [0002] With the increasing emergence and spread of antibiotic-resistant bacteria, a key factor in the proper treatment of infections is the ability to quickly and robustly characterize the antibiotic susceptibility of infectious species in order to ensure the use of effective antibiotics and reduce the need for broad-spectrum drugs. Resistance of bacterial pathogens to antibiotics is currently detected by phenotyping in the presence and absence of antibiotics, or by genotyping genetic markers associated with previously observed phenotypic resistance. [0003] Phenotypic antibiotic susceptibility testing (AST) is usually based on the detection of differential bacterial growth with and without antibiotics in liquid culture or on solid agar plates. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06B81B1/00C12M1/20G01N33/487
CPCC12Q1/18G01N33/5011G01N33/5008
Inventor J·逸夫M·瑞德O·巴尔特金M·洛夫马P·哈马E·阿姆塞伦M·奥尔森O·厄曼
Owner ASTREGO DIAGNOSTICS AB
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