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A preparation method of electrochemical impedance electrode for detecting bacteria

An electrochemical and electrode technology, applied in the field of polymers and electrochemistry, can solve the problems that limit the wide application of biosensors, the instability of biomolecules, the complexity of microelectrode arrays, etc., and achieve the improvement of poor water solubility and simple and mature reaction mechanism , The effect of simple preparation process

Inactive Publication Date: 2020-09-04
TIANJIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the complexity of microelectrode arrays, the instability of biomolecules and the high cost of both greatly limit the widespread application of this type of biosensor

Method used

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  • A preparation method of electrochemical impedance electrode for detecting bacteria
  • A preparation method of electrochemical impedance electrode for detecting bacteria
  • A preparation method of electrochemical impedance electrode for detecting bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Put 2 g of polyhexamethylene guanidine, 20 mg of 3,4,9,10-perylenetetracarboxylic dianhydride, and 8 mL of n-butanol into a 50 mL single-necked flask. The reaction system was stirred and reacted at 120° C. for 16 h under the protection of nitrogen. The crude product was obtained after ethanol dissolution, centrifugation, rotary evaporation, isopropanol precipitation, etc., and then vacuum-dried at 70°C for 24 hours to obtain the final product PBI20-PHMG (the initial dosage of PDA was 20 mg). The specific synthetic route is attached figure 1 .

[0043] The structure of the product was obtained by infrared (see attached figure 2 ), NMR (see attached image 3 ) to characterize.

Embodiment 2

[0045] Put 2 g of polyhexamethylene guanidine, 30 mg of 3,4,9,10-perylenetetracarboxylic dianhydride, and 8 mL of n-butanol into a 50 mL single-necked flask. The reaction system was stirred and reacted at 120° C. for 16 h under the protection of nitrogen. The crude product was obtained after ethanol dissolution, centrifugation, rotary evaporation, isopropanol precipitation, etc., and then vacuum-dried at 70°C for 24 hours to obtain the final product PBI30-PHMG (the initial dosage of PDA was 30 mg).

Embodiment 3

[0047] Put 2 g of polyhexamethylene guanidine, 40 mg of 3,4,9,10-perylenetetracarboxylic dianhydride, and 8 mL of n-butanol into a 50 mL single-necked flask. The reaction system was stirred and reacted at 120° C. for 16 h under the protection of nitrogen. The crude product was obtained after ethanol dissolution, centrifugation, rotary evaporation, isopropanol precipitation, etc., and then vacuum-dried at 70°C for 24 hours to obtain the final product PBI40-PHMG (the initial dosage of PDA was 40 mg).

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Abstract

The invention discloses a preparation method of an electrochemical impedance electrode for detecting bacteria. By taking polyhexamethylene guanidine and 3,4,9,10-perylenetetracarboxylic dianhydride asraw materials and through a simple anhydride ammonolysis reaction, a novel macromolecule bactericidal disinfectant, namely perylene polyhexamethylene guanidine is synthesized. Through the product, not only is the antibacterial activity of the polyhexamethylene guanidine improved, but also the poor water solubility of perylene derivatives is significantly overcome. Aiming at the seven kinds of polyhexamethylene guanidine with different perylene contents, the relationship between the antibacterial activity of the polyhexamethylene guanidine and the perylene grafting amount of a polymer is studied. Finally, the electrode which can be used for bacterial detection is prepared by combining the perylene polyhexamethylene guanidine with a graphene oxide material. By testing the change of an alternate-current impedance value, it is observed that the electrode can effectively capture and kill the bacteria, is high in sensitivity and can well detect escherichia coli and staphylococcus aureus suspensions with the low concentrations.

Description

technical field [0001] The present invention relates to the fields of macromolecules and electrochemistry, synthesizes a new high-efficiency antibacterial agent polyhexamethyleneguanidinyl perylene, combines it with graphene oxide materials and applies it to the field of electrochemistry, designs an effective detection of bacteria and Impedance electrode sensor with high sensitivity. Background technique [0002] Disinfectants are a class of chemicals that inhibit and kill microorganisms and are widely used in hospitals, homes, factories and other fields. Environmentally-friendly high molecular polymer - Polyhexamethyleneguanidine (PHMG) has superior bactericidal activity, can generate ionization in aqueous solution, and the hydrophilic part contains strong positive charge, which can adsorb various bacteria that are usually negative charge , viruses, enter the cell membrane, inhibit the synthesis of liposomes in the membrane, cause cell apoptosis, and achieve the best bacte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/02C08G73/00C23C26/00
CPCC08G73/00C23C26/00G01N27/02G01N27/327
Inventor 王亮朱灵利
Owner TIANJIN UNIVERSITY OF TECHNOLOGY
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