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How to use electronic rays and/or X -rays to irrigate mammalian cell groups

A technology of mammals and electron rays, which is applied to animal cells, vertebrate cells, and treatment of microorganisms with electricity/wave energy, and can solve problems such as difficult control of attenuation radiation

Active Publication Date: 2022-08-09
FRAUNHOFER GESELLSCHAFT ZUR FOERDERUNG DER ANGEWANDTEN FORSCHUNG EV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0019] Additionally, the technical implementation of gamma radiation of cellular products can sometimes be a problem, as it requires stringent shielding and safety measures, and attenuating radiation is often technically more difficult to control (WO 2015 / 011265 A1)

Method used

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  • How to use electronic rays and/or X -rays to irrigate mammalian cell groups
  • How to use electronic rays and/or X -rays to irrigate mammalian cell groups
  • How to use electronic rays and/or X -rays to irrigate mammalian cell groups

Examples

Experimental program
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Effect test

Embodiment Construction

[0171] method

[0172] At 37°C, 5% CO2 and 100% humidity, with the addition of 20% FCS (Fisher Scientific, Sveert), 2 mM glutamine (Fisher Scientific, Sveerte), 100 mM HEPES (Fisher Scientific, Sveerte) , 1% MEM amino acids (Fisher Scientific, Svelte), 100 U / ml penicillin-streptomycin (LifeTechnologies, Karlsruhe) and 100 U / ml IL-2 (ReproTech, Hamburg, #200-02) in RPMI 1640 Suspension cell lines NK-92 (Natural Killer Lymphoma, DSMZ#: ACC 488) and KHYG-1 (Natural Killer Lymphoma, DSMZ#: ACC 725) were expanded in medium (Life technologies, Karlsruhe); Obtained from the German Collection of Microorganisms and Cell Cultures [DSMZ], Braunschweig, Germany). Keep the number of cells used for culture or expansion at 0.2-1 x 10 6 cells / ml medium.

[0173] For each experiment, irradiate 70 μl of 1 x 10 7 cells / ml cell suspension. For this purpose, cell number and viability were determined by trypan blue counting before irradiation. Specifically, 10 μl of cell suspension (see abo...

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Abstract

The present invention relates to a method for irradiating a mammalian cell population with electron rays and / or X-rays, the mammalian cell population comprising at least one target mammalian cell, characterized in that: 1) using electron rays and / or X-rays Radiation in vitro irradiates a composition comprising a mammalian cell population, wherein the mammalian cell population comprises at least one target mammalian cell, and at a dose rate of 5 Gy / sec to 10 7 Gy / sec; 2) optionally isolating or enriching viable mammalian cells of interest from a population of mammalian cells, and agents obtainable therefrom and uses thereof.

Description

technical field [0001] The present invention relates to a method for irradiating a mammalian cell population with electron rays and / or X-rays, wherein said cell population comprises at least one target mammalian cell, characterized in that: i) with electron beams and / or X-rays In vitro irradiation of a composition comprising a mammalian cell population, wherein the mammalian cell population comprises at least one target mammalian cell, and at a dose rate of 5 Gy / sec to 10 7 In the range of Gy / sec; ii) optionally isolating or enriching viable mammalian cells of interest from a population of mammalian cells, and agents obtainable therefrom and uses thereof. Background technique [0002] Cell therapy can be used to treat a wide variety of diseases. Particularly promising at present is the research level and clinical application of cell therapy in the field of cancer treatment (Melief et al., 2011). [0003] However, a number of problems have arisen with the use of this treatm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/10A61K35/00C12N5/0783
CPCC12N5/0646C12N2529/10A61K41/10A61K35/17C12N13/00
Inventor F-H·勒格纳S·乌尔贝特J·布克哈特J·波蒂略J·舍恩费尔德
Owner FRAUNHOFER GESELLSCHAFT ZUR FOERDERUNG DER ANGEWANDTEN FORSCHUNG EV
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