Intestinal prebiotics oudemansiella raphanipes polysaccharide ORP-1 as well as preparation method and application thereof

A technology of ORP-1, Polysaccharide of Galleria vulgaris, applied in the direction of food science, etc., to achieve the effect of remarkable efficacy, broad market application prospect, and promotion of proliferation

Active Publication Date: 2019-12-31
ANHUI UNIVERSITY
View PDF5 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the researches focus on the optimization of the polysaccharide extraction process and the optimization of the mycelium liquid fermentation medium, but there are few reports on the activity of the polysaccharide

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Intestinal prebiotics oudemansiella raphanipes polysaccharide ORP-1 as well as preparation method and application thereof
  • Intestinal prebiotics oudemansiella raphanipes polysaccharide ORP-1 as well as preparation method and application thereof
  • Intestinal prebiotics oudemansiella raphanipes polysaccharide ORP-1 as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The new intestinal prebiotic of this embodiment is the polysaccharide ORP-1 of Gallus pilosulae, and its molecular weight is 2.4×10 4 Da, its monosaccharide composition is mannose in molar ratio: glucose: galactose: fucose=1.48:1.00:29.65:8.22; its preparation steps are as follows:

[0025] Using 600mg of crude polysaccharide from Gallus fruticosa with a purity of not less than 85% as raw material, carry out step-by-step alcohol precipitation with ethanol concentrations of 40% and 50%; the precipitate is dried, weighed, and dissolved, then placed in DEAE-52 cellulose Chromatographic column, the sample loading conditions are 100mg crude polysaccharide dissolved in 1mL distilled water, flow rate 1mL / min, to obtain a water-eluting polysaccharide solution; then concentrated, freeze-dried, weighed, dissolved and placed in superdex-75 molecular sieve gel chromatography The loading conditions for the column were 50 mg polysaccharide dissolved in 300 μL distilled water at a flo...

Embodiment 2

[0028] The new intestinal prebiotic of this embodiment is the polysaccharide ORP-1 of Gallus pilosulae, and its molecular weight is 2.4×10 4 Da, its monosaccharide composition is mannose in molar ratio: glucose: galactose: fucose=1.48:1.00:29.65:8.22; its preparation steps are as follows:

[0029] Using 600mg of crude polysaccharide from Gallus fruticosa with a purity of not less than 85% as raw material, carry out step-by-step alcohol precipitation with ethanol concentrations of 40% and 60%; the precipitate is dried, weighed, and dissolved, then placed in DEAE-52 cellulose Chromatographic column, the sample loading conditions are 100mg crude polysaccharide dissolved in 1mL distilled water, flow rate 1mL / min, to obtain a water-eluting polysaccharide solution; then concentrated, freeze-dried, weighed, dissolved and placed in superdex-75 molecular sieve gel chromatography The loading conditions for the column were 50 mg polysaccharide dissolved in 300 μL distilled water at a flo...

Embodiment 3

[0032] The new intestinal prebiotic of this embodiment is the polysaccharide ORP-1 of Gallus pilosulae, and its molecular weight is 2.4×10 4 Da, its monosaccharide composition is mannose in molar ratio: glucose: galactose: fucose=1.48:1.00:29.65:8.22; its preparation steps are as follows:

[0033]Using 600mg of crude polysaccharide from Gallus fruticosa with a purity of not less than 85% as raw material, carry out step-by-step alcohol precipitation with ethanol concentrations of 40% and 70%; the precipitate is dried, weighed, and dissolved, then placed in DEAE-52 cellulose Chromatographic column, the sample loading conditions are 100mg crude polysaccharide dissolved in 1mL distilled water, flow rate 1mL / min, to obtain a water-eluting polysaccharide solution; then concentrated, freeze-dried, weighed, dissolved and placed in superdex-75 molecular sieve gel chromatography The loading conditions for the column were 50 mg polysaccharide dissolved in 300 μL distilled water at a flow...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to view more

Abstract

The invention discloses an intestinal prebiotics oudemansiella raphanipes polysaccharide ORP-1 as well as a preparation method and application thereof. The intestinal prebiotics is the oudemansiella raphanipes polysaccharide ORP-1, and is obtained by using oudemansiella raphanipes polysaccharide as a raw material through the steps of stepwise alcohol precipitation, DEAE-52 cellulose column chromatography and superdex-75 molecular sieve gel column chromatography separation purification; and a molecular weight of the compound is 2.4 x 10<4> Da, and the molar ratio of monosaccharide composition of mannose to glucose to galactose to fucose is 1.48:1.00: 29.65:8.22. In the process of simulating oral, stomach and small intestine digestion in vitro, the novel intestinal prebiotics has the property of resisting digestion, but in the process of simulating fermentation of human feces in vitro, the novel intestinal prebiotics can be fermented and utilized by fecal microorganisms, and promote theproliferation of bifidobacterium bifidus and lactobacillus acidophilus of the intestinal probiotics.

Description

technical field [0001] The invention belongs to the technical field of prebiotic food, and in particular relates to an intestinal prebiotic polysaccharide ORP-1 from Gallus fruticosa black skin and its preparation method and application. [0002] technical background [0003] P. black skin fungus, scientific name Oudemansiella raphanipes (Oudemansiella raphanipes). The fungus has high edible and medicinal value, and is also one of the traditional medicinal fungi in my country. It is favored by people because of its fresh and mellow, tender meat, white as jade, unique taste, edible both raw and cooked, dual-purpose food and medicine, and rich nutrition. Compared with other fungal polysaccharides, there are less researches on the polysaccharides of Pollynus versicolor. Most of the researches focus on the optimization of the polysaccharide extraction process and the optimization of the mycelium liquid fermentation medium, but there are few reports on the activity of the polysa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A23L33/125
CPCA23L33/125C08B37/0003C08B37/006
Inventor 潘文娟姚程颖王志石露露张欣沈轩陈彦
Owner ANHUI UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap