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Tachypleus tridentatus SSR primer group and application thereof

A primer set and variety technology, applied in the direction of recombinant DNA technology, biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as lack of scientific basis

Active Publication Date: 2019-12-31
BEIBU GULF UNIV
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

There are relatively few technical methods for studying Chinese horseshoe crabs, and reports on the study of Chinese horseshoe crabs from the separation of microsatellite markers (Microsatellite DNA) are relatively rare. Due to the defects in the protection of Chinese horseshoe crab species, the environmental resources suitable for the growth of Chinese horseshoe crabs are declining day by day. Therefore, At present, there are very few samples used for the study of Chinese horseshoe crabs, and it is difficult to accurately reflect the true situation of the genetic diversity of Chinese horseshoe crabs; it also shows that the genetic diversity of Chinese horseshoe crabs is currently in its infancy, and these results cannot be used for the protection of Chinese horseshoe crabs. To provide a strong scientific basis, in order to conduct comprehensive and effective research on Chinese horseshoe crab, it is necessary to carry out the research work of this application, and to develop new research ideas for the SSR marker of Chinese horseshoe crab

Method used

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  • Tachypleus tridentatus SSR primer group and application thereof
  • Tachypleus tridentatus SSR primer group and application thereof
  • Tachypleus tridentatus SSR primer group and application thereof

Examples

Experimental program
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Embodiment

[0024] 1. Experimental method:

[0025] 1. Extraction method of limulus genomic DNA:

[0026] The blood tissue genomic DNA extraction kit (DP318-02) and marine animal tissue genomic DNA extraction kit (DP324-03) produced by Tiangen Biochemical Technology (Beijing) Co., Ltd. were used to extract the genomic DNA of horseshoe crab blood and horseshoe crab muscle. , And dilute to 20ng·μL -1 Store in the refrigerator at -20℃ for later use.

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Abstract

The invention relates to the technical field of molecular markers, in particular to a tachypleus tridentatus SSR primer group and application thereof. According to the invention, DNA extraction is carried out on tachypleus tridentatus, corresponding SSR primers are designed by sequencing and assembly, a plurality of tachypleus tridentatus genome DNAs in the Beibu gulf are used as materials, the designed SSR primers are verified, the obtained 164 pairs of SSR primers can be used for amplification in the tachypleus tridentatus, 20 pairs of polymorphic SSR primers are selected to carry out molecular genetic marking on the tachypleus tridentatus, a plurality of tachypleus tridentatus sample genes in the Beibu gulf area can be distinguished, different varieties of tachypleus tridentatus in theBeibu gulf area are subjected to clustering analysis, and a new thought is provided for research of genetic breeding, gene localization, species genetic relationship identification, tachypleus tridentatus genetic resource protection and the like in the future.

Description

【Technical Field】 [0001] The invention relates to the technical field of molecular markers, in particular to a Chinese horseshoe crab SSR primer set and its application. 【Background technique】 [0002] Chinese horseshoe crab has high economic and medical value. The horseshoe crab reagent made from its blood can be used to detect bacterial endotoxin. Its meat, shell and tail can be used as medicine. It is used in Guangxi, Hainan, Guangdong, Hong Kong, Fujian and Taiwan in my country. , Zhejiang and other coastal provinces and regions are distributed. In recent years, due to man-made or non-human factors such as overfishing and environmental degradation, the number of horseshoe crabs in China has decreased significantly and is on the verge of extinction. For a species, the higher its genetic diversity, the stronger its potential adaptability to changes in the living environment. The essence of Chinese horseshoe crab species protection lies in the protection of its genetic diversity...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11C12Q1/6869G06K9/62
CPCC12Q1/6888C12Q1/6869C12Q2600/124C12Q2600/156G06F18/23C12Q2531/113
Inventor 王鹏良廖永岩许尤厚吴海萍朱鹏吴双成黄志兴苏洁霞李艺谢雅榆邓洁梅
Owner BEIBU GULF UNIV
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