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Group of SSR molecular markers based on camellia japonica transcriptome and application of SSR molecular markers in camellia plants

A technology of molecular markers and transcriptomes, applied in the biological field, can solve the problems that the Camellia plants cannot be widely used, the application effect of the Camellia plants cannot be expected, and the average PIC value is low, so as to achieve high polymorphism, high specificity, high metastatic effect

Pending Publication Date: 2020-11-13
WUHAN ACADEMY OF AGRI SCI
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Problems solved by technology

In the cluster analysis, although the study separated the materials from Camellia (C.japonica) and Camellia oleifera (C.oleifera) well, it is not possible to predict the effect of its application in Camellia.
[0010] Although the above prior art involves the development and application of SSR markers, its average PIC values ​​are all low, and cannot be widely used in Camellia plants

Method used

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  • Group of SSR molecular markers based on camellia japonica transcriptome and application of SSR molecular markers in camellia plants
  • Group of SSR molecular markers based on camellia japonica transcriptome and application of SSR molecular markers in camellia plants
  • Group of SSR molecular markers based on camellia japonica transcriptome and application of SSR molecular markers in camellia plants

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Embodiment

[0035] The inventors of the present application previously obtained high-quality leaf transcriptomes of Camellia japonica (C. japonica) through RNA sequencing technology based on the Illumina platform. These sequence data provide a good resource for the development of Camellia SSR markers. The purpose of this study is to: 1) develop a set of SSR molecular markers based on the Camellia transcriptome; 2) determine the generality and polymorphism of these SSR markers in Camellia species; 3) reveal the genetic relationship and genetic structure of Camellia . The results of this study will provide important information for genetic improvement studies such as taxonomic studies, genetic diversity analysis, molecular marker-assisted breeding, and SSR-based genetic linkage map construction of Camellia plants.

[0036] 1. Transcriptome SSR characteristics of Camellia japonica leaves

[0037] SSRs are highly abundant in the assembled Camellia japonica leaf transcriptome. This applicat...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a group of SSR molecular markers based on camellia japonica transcriptome and application of the SSR molecular markers in camellia plants. 28854 pairs of SSR markers are developed in total, 13323 pairs of SSR primers are developed in total, 111 pairs of polymorphic SSR markers are further obtained, account for 79.85% of effective amplification primers and are far higher than the level of the prior art; meanwhile, the PIC value range of 111 pairs of polymorphism markers is 0.15-0.86, and the average value is 0.59 and is far higher than the existing level. Data further obtained by 27 pairs of polymorphic markers reveals a real genetic relationship and a group structure among 89 camellia varieties derived from camellia. In conclusion, the research result can provide high-quality SSR marker resources for genetic improvement research of camellia plants in the future.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a group of SSR molecular markers based on the transcriptome of Camellia japonica and its application in plants of the genus Camellia. Background technique [0002] The genus Camellia belongs to the family Theaceae and is widely distributed in southern my country and Southeast Asia. Several species in the Camellia genus are economically important. Such as camellia (C.japonica), Yunnan camellia (C.reticulata), Nujiang red camellia (C.saluenensis) and tea plum (C.sasanqua), are famous for their charming flowers; and tea tree (C.sinensis) is famous for its young leaves that are used to produce tea; the seeds of some species, such as Camellia oleifera (C. oleifera) and Camellia oleifera (C. semiserrata), are used to produce high-quality edible and medicinal oils. [0003] The genus Camellia includes more than 300 species and is the largest genus in the family Theaceae. Amon...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 李清源许林陈宝林傅劭付田静向琮琳杨守坤陈卫东魏鸣谢焰锋
Owner WUHAN ACADEMY OF AGRI SCI
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