The invention provides a retiform biological filling device and relates to a water root position cleaning treatment biological filling device. The retiform biological filling device comprises a filling grid (1), a combination filling unit (2), a rope (3), a float ball (4), a sleeve (5) and a dead lever (6); the combination filling unit (2) is fixedly connected with the grid crunode of the filling grid (1), the upper end of the filling grid (1) is connected with the float ball (4) by the rope (3), the two ends of the filling grid (1) is fixedly connected with the sleeve (5) by fixed boundary lines, when in use, the dead lever (6) is inserted in the sleeve (5), and fixation to the retiform biological filling is realized. The retiform biological filling of the invention has the advantages of simple structure, high specific surface area, convenient processing and transportation, not jamming, not caking, long service life, simple installation, convenient management and maintenance, strong ability of anti impulsion of water flow, and the like, which can be used in root position cleaning treatment of natural water such as riverway, lake and reservoir, and artificial water.