Preparation of acid soluble pulse protein hydrolyzates with little or no astringency and pulse protein hydrolyzates of improved amino acid score
A technology of soy protein and proteolytic enzyme, applied in the field of soy protein hydrolyzate, can solve the amino acid score limitation and use limitation of pea protein, U.S. Patent Publication No. 2011/0274797), 2012 published on November 10, 2012 13/556357 filed Jul. 24, 2013 (US Patent Publication No. 2013/00189408 published Jul. 25, 2013), 13/642003 filed Jan. 7, 2013 (May 23, 2013) Published U.S. Patent Publication No. 2013/0129901) and issues filed on February 11, 2016
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Embodiment 1
[0042] This example describes the preparation of a soy protein hydrolyzate from a neutral dry powdered soy protein product according to one embodiment of the method of the present invention.
[0043] 36 kg of yellow pea protein concentrate was added to 600 L of reverse osmosis purified water at ambient temperature and stirred for 10 minutes to provide an aqueous protein solution. A portion of the suspended solids was removed by centrifugation using a decanter centrifuge, and a protein solution having a protein content of 2.34% by weight was collected. The pH of the protein solution was lowered to 3.07 by the addition of HCl solution (concentrated HCl diluted with an equal volume of water) and the solution was then warmed to 50°C for 10 minutes before centrifugation using a disc centrifuge. 519 L of acidified protein solution and 77.44 kg of acid insoluble solid material were collected.
[0044]The pH of the acidified protein solution with a protein content of 0.82 wt% was adj...
Embodiment 2
[0051] This example describes another example of preparation of a soy protein hydrolyzate from a neutral dry powdered soy protein product according to one embodiment of the method of the present invention.
[0052] 36 kg of yellow pea protein concentrate was added to 600 L of reverse osmosis purified water at ambient temperature and stirred for 10 minutes to provide an aqueous protein solution. A portion of the suspended solids was removed by centrifugation using a decanter centrifuge, and a protein solution having a protein content of 2.72% by weight was collected. The pH of the protein solution was lowered to a pH of about 3 by adding HCl solution (concentrated HCl diluted with an equal volume of water), the solution was warmed to 50°C for 10 minutes, and then centrifuged using a disc centrifuge. 474.8 L of acidified protein solution and 85.85 kg of acid-insoluble solid material were collected.
[0053] The acidified protein solution with a protein content of 1.66 wt% was d...
Embodiment 3
[0058] This example describes another example of preparation of a soy protein hydrolyzate from a neutral dry powdered soy protein product according to one embodiment of the method of the present invention.
[0059] 96 kg of yellow pea protein powder was added to 600 L of reverse osmosis purified water at ambient temperature and stirred for 10 minutes to provide an aqueous protein solution. A portion of the suspended solids was removed by centrifugation using a decanter centrifuge, and a protein solution having a protein content of 3.94% by weight was collected. The pH of the protein solution was lowered to 2.05 by the addition of HCl solution (concentrated HCl diluted with an equal volume of water), the solution was warmed to 50°C for 10 minutes, and then centrifuged using a disc centrifuge. 524 L of acidified protein solution and 83.98 kg of acid insoluble solid material were collected.
[0060] The acidified protein solution with a protein content of 3.46 wt% was reduced to...
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