Nanoprobe and production method and application thereof

A nano-probe and organic technology, applied in the field of nano-probes, can solve problems such as short excitation wavelength, background interference, difficulty in accurately detecting albumin content, etc., achieve low detection limit, wide detection range, enhanced fluorescence intensity and photostability sexual effect

Active Publication Date: 2020-01-03
SHENZHEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a nanoprobe and its preparation method and application, aiming to solve the problem that the existing fluorescent probe molecules have short excitation wavelengths, are prone to background interference, and are difficult to accurately detect albumin. content problem

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  • Nanoprobe and production method and application thereof
  • Nanoprobe and production method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0049] (1) Heptamethine dye is , according to the reaction formula

[0050] Preparation of cyanine organic matter; the specific preparation steps are: the heptamethine dye (100mg, 0.14mmol) and 4-(amino)phenyl 2,2,6,2-terpyridine (100mg, 0.31mmol) in anhydrous N , stirred in N-dimethylformamide (DMF) (4mL) at 90°C for 24 hours, cooled to room temperature, and spin-dried to remove the solvent, using dichloromethane / methanol (25:1 by volume) as eluent, After separation by silica gel column chromatography, cyanine organic compounds were obtained. The structural characterization data of the cyanine organic matter is: 1H NMR (600MHz, CDCl 3 )δ8.32(d, J=14.1Hz, 2H), 8.09(d, J=8.5Hz, 2H), 7.85(dd, J=19.1, 8.2Hz, 4H), 7.56(t, J=7.6Hz, 2H), 7.43(s, 2H), 7.34(d, J=8.8Hz, 2H), 7.28(m, 9H), 7.08(d, J=7.5Hz, 1H), 6.77(s, 1H), 6.24( d,J=14.1Hz,1H),5.58(s,2H),5.24(s,1H),2.52(s,4H),2.00(s,9H),1.65(s,2H),1.19(s,2H ); The molecular weight that flight mass spectrometry records is 1023.50...

Embodiment 2

[0054] Characterize the sensitivity of the nanoprobe (PNC) that embodiment 1 makes: prepare the aqueous solution of the nanoprobe (PNC) (15 μ M) that 3mL embodiment 1 makes; Bovine serum albumin (BSA) is dissolved in deionized Prepare the sample solution to be tested (55mg / mL) with water, then add 0-1.0% bovine serum albumin in the nanoprobe aqueous solution at a volume concentration interval of 0.1%, measure the fluorescence intensity by a fluorescence spectrophotometer, and the nanoprobe The change of fluorescence spectrum with the concentration of BSA is as follows Figure 5 As shown, it shows that the fluorescence intensity of the nanoprobe solution increases with the concentration of BSA. The nanoprobe (PNC) that embodiment 1 makes is the fluorescence intensity when fluorescence wavelength is 610nm and the change relationship with the concentration of BSA is as follows Figure 6 As shown, it can be seen that the two have a linear relationship, and the calculation shows t...

Embodiment 3

[0056] Characterize the specificity of the nanoprobe (PNC) prepared in Example 1: prepare the aqueous solution of 3mL nanoprobe (15 μ M), respectively starch, esterase, trypsin, transferrin, glutathione, spherical Protein, cholesterol, bovine serum albumin, human serum albumin biological sample were dissolved in deionized water respectively to prepare test sample solution (1.0mM), then each test solution of above-mentioned 5% was added in the nanoprobe solution, with Add the aqueous solution of the nanoprobe (15 μ M) of test solution as blank control experiment, adopt fluorescence spectrophotometer to measure fluorescence, the fluorescence intensity contrast situation of the PNC solution that contains different biological samples is as follows Figure 7 As shown, it can be seen that only two solutions of bovine serum albumin and human serum albumin interact with the nanoprobe to produce significant fluorescence, indicating that albumin can specifically bind to the nanoprobe to ...

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Abstract

The invention discloses a nanoprobe and a production method and application thereof. The nanoprobe is formed by a cyanine organic matter and an amphipathic substance which covers the cyanine organic matter, and a structural general formula of the cyanine organic matter is as shown in the specification, wherein R is CH3 or CH2CH3. According to the nanoprobe and the production method and applicationthereof, the cyanine organic matter which has a heptamethine cyanine dye structure with a large conjugated system and a terpyridyl structure with a rigid plane system and the amphipathic substance are used for forming the nanoprobe, and the fluorescence intensity and the photostability which are generated when the nanoprobe and albumin are bound are enhanced, so that the nanoprobe has the characters of being wide in detection range, high in sensibility and low in detection limit when detecting the albumin.

Description

technical field [0001] The invention relates to the technical field of nanoprobes, in particular to a nanoprobe and its preparation method and application. Background technique [0002] In clinical diagnosis, the quantitative detection of proteins and their biomarkers is very important for the diagnosis of diseases. Among various proteins, albumin (albumin, ALB) is the most abundant transport protein in blood, and has important biological functions, including promoting the transportation of various drugs, fatty acids and metabolites, and maintaining blood osmotic pressure. Generally speaking, the concentration of albumin in normal serum is 35-55g L -1 . The concentration of albumin in the blood can reflect the health status of an individual. For example, low concentrations of albumin in plasma, called hypoalbuminemia, may indicate liver and kidney disease or malnutrition associated with a low-protein diet. Conversely, albumin concentrations in normal urine are <30 mg·...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D401/14C09K11/06G01N21/64
CPCC07D401/14C09K11/06G01N21/6428G01N2021/6432G01N2021/6417G01N2021/6439C09K2211/1029C09K2211/1007
Inventor 黄鹏袁燕燕贺婷林静
Owner SHENZHEN UNIV
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