Heterotrophic nitrification-aerobic denitrification pseudomonad strain, pseudomonad seed liquid, preparation method of pseudomonad seed liquid and application of heterotrophic nitrification-aerobic denitrification pseudomonad strain

An aerobic denitrification and pseudomonas technology, applied in the field of microorganisms, can solve the problems of poor resistance to high carbon-nitrogen ratio and high-concentration nitrogen, and constraints, and achieve the effect of great application value

Active Publication Date: 2020-01-07
LONGYAN UNIV
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Problems solved by technology

However, the currently disclosed HN-AD strains have poor tolerance to high carbon-to-nitrogen ratios and high concentrations of nitrogen, which restricts the p

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  • Heterotrophic nitrification-aerobic denitrification pseudomonad strain, pseudomonad seed liquid, preparation method of pseudomonad seed liquid and application of heterotrophic nitrification-aerobic denitrification pseudomonad strain
  • Heterotrophic nitrification-aerobic denitrification pseudomonad strain, pseudomonad seed liquid, preparation method of pseudomonad seed liquid and application of heterotrophic nitrification-aerobic denitrification pseudomonad strain
  • Heterotrophic nitrification-aerobic denitrification pseudomonad strain, pseudomonad seed liquid, preparation method of pseudomonad seed liquid and application of heterotrophic nitrification-aerobic denitrification pseudomonad strain

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Embodiment

[0062] A heterotrophic nitrification-aerobic denitrification Pseudomonas strain of the present invention, which is preserved in Guangdong Microbial Culture Collection Center (abbreviation: GDMCC), and the preservation number is GDMCC NO: 60339 strain. The Pseudomonas GDMCC NO: 60339 is named as Pseudomonas indoloxydans LJ9 (Pseudomonas indoloxydans LJ9).

[0063] 1) Separation, screening and purification of strains

[0064] The Pseudomonas LJ9 of the above-mentioned present embodiment is mainly screened by the following methods:

[0065] 1.1) Enrichment culture: 50 mL of activated sludge collected from Longjin Sewage Treatment Plant in Longyan City, Fujian Province was added to 100 mL of DM medium, and 2-4 sterile glass beads were added to the medium to break up the sludge. 30°C, 150rpm shaker shaking continuous culture for 5 days. The above bacterial suspension was transferred to a new DM medium with a 10% inoculum amount, and culture was repeated twice in this way to obtai...

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Abstract

The invention discloses a heterotrophic nitrification-aerobic denitrification pseudomonad strain, seed liquid obtained through culturing the strain, and a culture method. The strain is pseudomonad LJ9(pseudomonas indoloxydans LJ9), and is preserved in Guangdong culture collection center, the preservation time is 21 March 2018, and the preservation number is GDMCC NO:60339. The pseudomonad strainhas the capacity for resisting high carbon-nitrogen ratio and resisting high nitrate nitrogen and nitrite nitrogen, and has potential application value in the treatment problem of high-concentration organic waste water.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a heterotrophic nitrification-aerobic denitrification Pseudomonas strain, a seed solution, a preparation method and application thereof. Background technique [0002] In recent years, the problem of water pollution has become increasingly serious. Nitrogen is an important pollution factor in water. Excessive nitrogen flowing into water can easily cause eutrophication of water, cause insufficient dissolved oxygen in water, lead to deterioration of water quality, and change water ecology. The environment, endangering the survival of aquatic organisms, is extremely harmful to the environment and human health. Nitrogen exists in water bodies in the form of organic nitrogen and inorganic nitrogen. The main source of organic nitrogen is domestic sewage, agricultural nitrogen fertilizer and industrial wastewater; Nitrate nitrogen, of which nitrate nitrogen can be converted into ...

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F101/16C12R1/38
CPCC12N1/20C02F3/34C02F2101/16C02F2101/163C02F2101/166C12R2001/38C12N1/205Y02W10/10
Inventor 孙巍夏春雨洪维祎宋玉文杨雅玲苏全榕阙才英魏清斯易艳玲宋雅坚林美琴
Owner LONGYAN UNIV
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