Process method for coating protein with magnetic beads

A process method and magnetic bead technology, which is applied in the field of magnetic bead-coated protein technology, can solve the problems of restricting the wide application of the process, prone to agglutination, low blank, etc., to avoid sticking cover/sticking wall loss, linear light value The effect of low blank and small CV

Inactive Publication Date: 2020-01-10
DIRUI MEDICAL TECH CO LTD
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Combined with the actual application results, Tosyl magnetic beads can generally obtain a higher coating rate, but because the functional groups are relatively hydrophobic, they are prone to aggregation; in contrast, carboxyl magnetic beads have the advantage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Process method for coating protein with magnetic beads
  • Process method for coating protein with magnetic beads
  • Process method for coating protein with magnetic beads

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Step 1: Replacement: Add 2.5mg of antibody / antigen to ultrafiltration centrifuge tubes, centrifuge at 5°C and 6000g upright for 3 times, and make up carboxyl magnetic bead coating buffer (pH6.5, 15mM MES, Contain 50mM NaCl), centrifuge at 35000g, recover the substance to be labeled after replacement, and use carboxyl magnetic bead coating buffer to set the concentration to 1mg / mL;

[0052] Step 2: Washing of magnetic beads: Take 210mg of magnetic beads (M-270Carboxylic Acid superparamagnetic beads provided by Dynal, the particle size of which is 2.8 μm) and add them to a 40mL glass bottle, use TBST buffer solution, wash 3 times with magnetic collection, and finally After discarding the supernatant once, resuspend to 10mg / mL with carboxyl magnetic bead coating buffer, and set aside;

[0053] Step 3: Take the resuspended magnetic bead bottle (containing 15 mg magnetic beads) after cleaning, place it on a 360 mixer, use horizontal swing mixing method, select mode "--", amp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention provides a process method for coating protein with magnetic beads, and belongs to the technical field of processes for coating protein with magnetic beads. The method comprises the following steps of: firstly, carrying out antigen/antibody replacement to obtain a replaced to-be-coated object; cleaning the magnetic beads; taking a cleaned resuspended magnetic bead bottle, placing theresuspended magnetic bead bottle on a 360-degree uniform mixing instrument, and premixing the magnetic beads with the replaced to-be-coated object in a horizontal swing uniform mixing manner, the concentration of the magnetic beads being 5mg/mL; adding eDC and NHS into the premixed magnetic beads, putting the magnetic beads on the 360-degree uniform mixing instrument for activation, wherein the mass ratio of EDC to the magnetic beads is 1:10; and adding a confining liquid, collecting magnetism to remove supernatant, and performing cleaning to obtain magnetic beads coated protein. According tothe process method, the protein coating efficiency can be improved, and the test performance of the corresponding kit is improved.

Description

technical field [0001] The invention belongs to the technical field of magnetic bead coating protein technology, and in particular relates to a magnetic bead coating protein process method. Background technique [0002] Chemiluminescent Immunoassay (CLIA) is a leading analytical technique with high sensitivity, high efficiency and high repeatability for detecting trace antigens / antibodies in samples by combining immune reaction and chemiluminescence reaction. The most common mode in this technique is to select a pair of antibodies / antigens that can be combined with the sample to be tested, one of which is coated on a solid phase carrier, and the other is labeled with a luminescent substance. At the same time, the antigen / antibody in the sample is combined to form a sandwich structure (sandwich method); at the same time, the methodology of CLIA also includes competition method, indirect method and capture method. [0003] The above methods all involve a process of coating th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/543
CPCG01N33/5434
Inventor 齐琳何浩会王麒于猛高威
Owner DIRUI MEDICAL TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap