A real-time tracking irradiation device for experimental mice

Abstract

The invention discloses a method for real-time tracking and irradiation of experimental mice. S1, using a camera to acquire an image of the mouse to be irradiated, judging the current posture of the mouse, and determining the area of ​​the mouse's head in the image; S2, acquiring a small The mouse image after the mouse position moves, according to the mouse head area determined in step 1, use the target tracking algorithm to determine the mouse head area in the new image; S3, calculate the pixel coordinates of the mouse head, according to the mouse head area Correct the pixel coordinates of the mouse head based on the current posture of the mouse, and then based on the pixel coordinates, calculate the displacement vector that needs the light to move according to the positional relationship between the camera and the light irradiation direction in the actual device; S4. According to the displacement vector in step S3, control the light to move Corresponding displacement and irradiation of the mouse's head; the invention corrects the position according to the mouse's posture, adjusts the light emitting position in real time so that the light moves with the mouse's movement, and solves the problem of the mouse's activity area being limited.

Description

technical field [0001] The invention relates to the intersecting fields of machinery, electronics and automation, in particular to an image positioning-based real-time tracking irradiation device and a method thereof. Background technique [0002] Optogenetics refers to a technology that combines optical and genetic means to activate or deactivate specific living tissues and cells. The theoretical approach is to incorporate opsin genes into brain neurons to create a tool to control neuronal excitation or inhibition with light. In optogenetics experiments, optical fibers are usually connected and fixed to the head of the mouse, so that light of a certain wavelength can be irradiated deep into the brain of the mouse. However, because the optical fiber hinders the movement of mice, this experimental method is not applicable in some studies on animal behavior. [0003] In 2017, some researchers proposed an experimental method of implanting a light-converting crystal into the h...

AI Technical Summary

Problems solved by technology

This method finds the position of the mouse head through image processing, corrects the position according to the posture of the mouse, calculates the actual spatial coordinates of the mouse head, and adjusts the position of the light emission in real time so that the light moves with the movement of the mouse. The problem of restricted activity area of ​​mice

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