Preparation method of rabbit pulmonary vascular model

A production method and technology of blood vessel models, applied in teaching models, educational tools, instruments, etc., can solve the problems of expensive, unfavorable specific distribution of pulmonary artery vessels, image artifacts, etc., and achieve the effect of low cost, simple and convenient learning and research

Inactive Publication Date: 2020-01-14
韦义萍
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  • Description
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  • Application Information

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Problems solved by technology

Clinically, pulmonary angiography is the gold standard for diagnosing the formation and distribution of PTE, but it is expensive and prone to image artifacts, which is not conducive to intuitively understanding the specific distribution of pulmonary arteries

Method used

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  • Preparation method of rabbit pulmonary vascular model
  • Preparation method of rabbit pulmonary vascular model

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Embodiment Construction

[0028] The present invention will be described in detail below in conjunction with the accompanying drawings.

[0029] Taking healthy New Zealand big-eared white rabbits with a body weight of 2.0-2.5kg as the test object, the method for making the rabbit pulmonary blood vessel model is as follows:

[0030] 1) Prepare the skin at the marginal ear vein of the rabbit, then inject 10mL of air along the marginal ear vein, and kill the rabbit;

[0031] 2) Use a scalpel to dissect the rabbit along the midline, and keep the rabbit lung and heart tissue;

[0032] 3) Flush the excess blood with 0.9% normal saline until the lung and heart tissue turns light red;

[0033] 4) Place the lung and heart tissue on the tray, select the gel pen refill for the catheter, rinse the ink in the pen, insert it into the superior vena cava, aorta, and main bronchus respectively, and use medical sutures to ligate and fix it respectively. Such as figure 1 shown;

[0034] 5) Select 30g of denture base ...

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Abstract

The invention discloses a preparation method of a rabbit pulmonary vascular model. After the lung and heart tissues of a rabbit are acquired, denture base resin solvents with different colors are infused to the pulmonary vein along the superior vena cava, are infused to the pulmonary artery along the aorta and are infused into the trachea along the main bronchus, and a denture base resin forming part in the lung tissue is left, so that the rabbit pulmonary vascular model is obtained. The method is low in cost, and people can be effectively helped to prepare the pulmonary vascular model for observing specific distribution of the pulmonary artery, the pulmonary vein, the trachea and the main bronchus and more simply and conveniently learning and studying the pulmonary vessels, thereby guiding acquisition of the lung tissue of the rabbit.

Description

technical field [0001] The invention relates to a method for making a model by using animal isolated tissues, in particular to a method for making a rabbit pulmonary blood vessel model. Background technique [0002] Pulmonary embolism (PTE) refers to the clinical syndrome of pulmonary circulation dysfunction caused by endogenous or exogenous emboli blocking the pulmonary artery. The mortality rate of untreated pulmonary embolism is as high as 30%. Early diagnosis and timely treatment can reduce the mortality rate by 2% to 10%. Therefore, finding sensitive indicators for early judgment of PTE formation and early thrombolysis can minimize the harm caused by PTE. [0003] Through animal experiments, the lung tissue of the animal model with intravenous catheterization in rabbits was collected for HE staining, which can be used to judge whether PTE is formed or not, and to further explore the expression of related indicators when PTE is formed. However, it is necessary to know ...

Claims

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Application Information

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IPC IPC(8): G09B23/28G09B23/36
CPCG09B23/28G09B23/36
Inventor 韦义萍覃燕廖海涛
Owner 韦义萍
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