Method for detecting amino acid decarboxylation ability of fish spoilage bacteria
A detection method, amino acid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganism measurement/inspection, etc., can solve problems such as the evaluation method of amino acid decarboxylation ability of fish spoilage bacteria that have not been seen
Active Publication Date: 2020-01-17
CHINA AGRI UNIV
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Problems solved by technology
[0006] However, so far, there have been no reports on the evaluation method of amino acid decarboxylation ability of fish spoilage bacteria
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[0044] The specific implementation manner of the present invention will be further described in detail below in conjunction with specific examples. The following examples are used to illustrate the present invention, but are not used to limit the protection scope of the present invention, and the protection scope of the present invention is as the criterion with claims.
[0045] Unless otherwise specified, the experimental reagents and materials used in the examples of the present invention are all commercially available.
[0046] If not specified, the technical means used in the embodiments of the present invention are conventional means well known to those skilled in the art.
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The invention discloses a method for detecting the amino acid decarboxylation ability of fish spoilage bacteria. The method includes dissolving a to-be-detected amino acid substrate by a phosphate buffer solution to obtain a to-be-detected amino acid substrate solution, adding a bacterial suspension containing to-be-detected spoilage bacteria into the to-be-detected amino acid substrate solution to serve as an evaluation group, adding the bacterial suspension containing the to-be-detected spoilage bacteria into the phosphate buffer solution to serve as a control group, adding sterile saline into the to-be-detected amino acid substrate solution to serve as a blank group, cultivating and extracting the evaluation group, the control group and the blank group under same conditions respectively, performing detection to obtain biogenic amine concentration C evaluation, C control and C blank, and performing calculation to obtain the decarboxylation ability of the spoilage bacteria to corresponding to-be-detected amino acids. The method can be widely applied to detection of the amino acid decarboxylation ability of various spoilage bacteria in a variety of fish, and is applicable to quantitative detection and evaluation of the decarboxylation ability of the spoilage bacteria to various specific amino acids and to evaluation of the biogenic amine generation ability of the fish spoilagebacteria.
Description
technical field [0001] The invention belongs to the field of storage and preservation technology and quality safety of aquatic products, and in particular relates to a method for detecting amino acid decarboxylation ability of fish spoilage bacteria. Background technique [0002] Fish meat has always been an important part of the daily diet of Chinese residents due to its rich nutrition, easy digestion, and delicious taste. Corruption and deterioration due to growth and reproduction will eventually affect the processing and consumption of fish meat products. [0003] Biogenic amines are important spoilage products and quality indicators in the process of fish spoilage. Some biogenic amines, such as putrescine and cadaverine, have a strong pungent odor, which can seriously affect the sensory quality of fish. Other biogenic amines, such as histamine, tyramine, and phenylethylamine, have certain physiological toxicity, which seriously threatens the health of consumers and foo...
Claims
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IPC IPC(8): C12Q1/02G01N30/88C12R1/38C12R1/01
CPCC12Q1/02G01N30/88G01N2333/21G01N2030/8818
Inventor 罗永康庄帅洪惠
Owner CHINA AGRI UNIV